{"product_id":"abcam-ab222519","title":"Abcam, ab222519, Anti-SNF5\/SMARCB1 antibody [EPR20189]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nAnti-SNF5\/SMARCB1 antibody [EPR20189] (ab222519) is a rabbit monoclonal antibody detecting SNF5\/SMARCB1 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF . Suitable for  Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR20189,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WB, IHC-P, ICC\/IF, Flow Cyt (Intra), IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-SNF5\/SMARCB1 antibody [EPR20189] (ab222519) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of SNF5\/SMARCB1?\u003cbr\u003e\nAnti-SNF5\/SMARCB1 [EPR20189] (ab222519) specifically detects a band for SNF5\/SMARCB1 (UniProt: Q12824) at a molecular weight of 44kDa.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR20189] also available for your convenience: ab222519, Carrier free -\u003cbr\u003e\nab233399\u003cbr\u003e\n, PE -\u003cbr\u003e\nab306033\u003cbr\u003e\n, APC -\u003cbr\u003e\nab306034\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab306035\u003cbr\u003e\n, Alkaline Phosphatase -\u003cbr\u003e\nab308748\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab309700\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab310067\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab310463\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab311993\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab312469\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321411\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSNF5\/SMARCB1 also known as INI1 or BAF47 is a member of the SWI\/SNF chromatin remodeling complex. It acts in modifying chromatin structure to regulate gene expression. SNF5\/SMARCB1 possesses a mass of approximately 47 kDa. The protein expresses ubiquitously in various tissues with notable presence in the nucleus. Through its mechanical activity in chromatin remodeling SNF5\/SMARCB1 facilitates transcriptional regulation and chromosomal stability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSNF5\/SMARCB1 acts as an integral component of the SWI\/SNF complex. This complex functions in altering nucleosome positioning on DNA impacting gene expression linked to cell cycle regulation differentiation and DNA repair. By working within this complex SNF5\/SMARCB1 helps in the activation or repression of specific gene sets based on cellular requirements displaying an essential role in maintaining normal cellular function. The interplay of SNF5\/SMARCB1 with other chromatin-modifying enzymes extends its influence across various genetic pathways.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of SNF5\/SMARCB1 in the cell cycle and transcription regulation pathways is acknowledged. It participates intimately in the p53 signaling pathway which is critical for cell cycle checkpoints and apoptotic responses. SNF5\/SMARCB1 engages with other proteins such as p53 and RB1 coordinating to maintain genomic integrity and proper transcriptional programming. These pathways spotlight the protein's influence in important cell fate decisions.\u003cbr\u003e\nSNF5\/SMARCB1 mutations or deletions associate closely with malignant rhabdoid tumors an aggressive pediatric cancer and schwannomatosis a disorder involving benign nerve sheath tumors. The loss of SNF5\/SMARCB1 disrupts the SWI\/SNF complex's function triggering unregulated cell proliferation and cancer progression. Within these disorders the SWI\/SNF complex itself which contains other subunits like BRG1 and BRM is also impacted highlighting SMARCB1's critical role in tumor suppression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855848001705,"sku":"ab222519","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab222519","provider":"Iright","version":"1.0","type":"link"}