{"product_id":"abcam-ab222942","title":"Abcam, ab222942, Total Collagen Assay Kit (Perchlorate-Free)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nTotal Collagen Assay Kit (Perchlorate-Free) ab222942 provides a quick and convenient method to detect collagen. - Detection limit as low as 0.5 µg of collagen - Does not require the use of hazardous perchloric acid for hydrolysates, removing any special handling and waste-disposal protocols\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Urine, Tissue Homogenate, Serum,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nRange:0.5 - 10 µg,\u003cbr\u003e\nAssay time:3h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nTotal collagen assay principle\u003cbr\u003e\nThe total collagen assay protocol is based on the total alkaline\/acid hydrolysis of samples to breakdown the collagen trihelix peptide into single amino acids and yield free Hydroxyproline. The released hydroxyproline is oxidized to form a reaction intermediate, which further in reaction forms brightly-colored chromophore that can be easily detected at OD 560 nm.\u003cbr\u003e\nThe total collagen assay uses an identical assay principle and assay components to our hydroxyproline assay kit\u003cbr\u003e\nab222941\u003cbr\u003e\n, except that the standard curve of this assay is constructed from collagen itself (and that collagen is treated in an identical manner to samples, undergoing alkaline hydrolysis at 120C). This makes this assay a more direct way to quantify collagen levels.\u003cbr\u003e\nThe assay employs a proprietary developer solution to accurately measure collagen in hydrolysates without the use of hazardous perchloric acid, removing the need for any special handling and waste-disposal protocols.\u003cbr\u003e\nTotal Collagen assay kit protocol summary:\u003cbr\u003e\n- Hydrolyze samples and standard with concentrated NaOH for 1 hr at 120°C\u003cbr\u003e\n- Cool on ice and neutralize with concentrated HCL\u003cbr\u003e\n- Centrifuge at 10,000g for 5 min to pellet debris\u003cbr\u003e\n- Add samples and standards to wells\u003cbr\u003e\n- Evaporate wells to dryness at 65°C\u003cbr\u003e\n- Add oxidation mix and incubate for 20 min\u003cbr\u003e\n- Add developer and incubate for 5 min\u003cbr\u003e\n- Add DMAB concentrate and incubate for 45 min at 65°C\u003cbr\u003e\n- Analyze absorbance at 560 nm with a microplate reader\u003cbr\u003e\nHow other researchers are using\u003cbr\u003e\nTotal Collagen Assay Kit (Perchlorate-Free) has been used in a variety of sample type including:\u003cbr\u003e\n- Mouse liver samples\u003cbr\u003e\n- Mesenchymal stem cell culture\u003cbr\u003e\nReferences:\u003cbr\u003e\n1- Chen X al. 2023\u003cbr\u003e\n2 Gousopoulou X et al. 2023\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K406 Total Collagen Assay Kit (Perchlorate-Free). K406-100 is the same size as the 100 test size of ab222942.\u003cbr\u003e\nCollagen is the most abundant insoluble protein found in the extracellular matrix and connective tissues. It can be found in skin, tendons, bone, cartilage, muscle, vitreous humor and ligaments, among other tissues. There are more than sixteen - well characterized types of collagens, but types I, II and III collagen comprise more than 80% content in mammals.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843548827817,"sku":"ab222942","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab222942","provider":"Iright","version":"1.0","type":"link"}