{"product_id":"abcam-ab223148","title":"Abcam, ab223148, Anti-Ribonuclease 3\/ECP antibody [EPR20357] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Ribonuclease 3\/ECP antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR20357,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab223148 is the carrier-free version of\u003cbr\u003e\nab207429\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRibonuclease 3 commonly known as Eosinophil Cationic Protein (ECP) is an enzyme with ribonuclease activity. Researchers sometimes refer to it as human RNase 3. The protein has a molecular mass of about 18 to 22 kDa and is highly expressed in eosinophils a type of white blood cell primarily involved in allergic responses and parasitic infections. ECP exhibits cytotoxic bactericidal and antiviral activities contributing to its diverse biological functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRibonuclease 3 plays a significant role in immune defense mechanisms leveraging its arginine-rich domain to interact with negatively charged microbial surfaces. This interaction allows ECP to participate in the destruction of pathogen cell walls. It is not part of a larger complex but interacts with cellular membranes making it an effective tool against a variety of pathogens. Whether acting through enzymatic activity or by disrupting membrane integrity ECP maintains a pivotal role in host defense.\u003cbr\u003e\nPathways\u003cbr\u003e\nECP influences processes in both innate and adaptive immune responses. It actively participates in the Th2 cell-mediated immune response which is critical for countering parasitic infections. This protein connects with other components like major basic protein and eosinophil peroxidase through these pathways forming an integrated network that boosts immune responses. Its presence in pathways linked to inflammation and immunity highlights its multifunctionality and importance.\u003cbr\u003e\nECP levels correlate with conditions such as asthma and eosinophilic esophagitis. In asthma elevated ECP levels indicate eosinophilic inflammation and airway hyperresponsiveness. Researchers have identified a connection between ECP and proteins like interleukin-5 which modulate eosinophil activity. Similarly in eosinophilic esophagitis increased ECP levels indicate ongoing inflammation and tissue damage providing insights into disease progression and severity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851763437737,"sku":"ab223148","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab223148","provider":"Iright","version":"1.0","type":"link"}