{"product_id":"abcam-ab223582","title":"Abcam, ab223582, Anti-EpCAM antibody [EPR20532-225]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-EpCAM antibody [EPR20532-225] (ab223582) is a rabbit monoclonal antibody detecting EpCAM in  Western Blot, Flow Cytometry, IP, IHC-P, ICC\/IF, mIHC . Suitable for  Human . - KO validated for confirmed specificity - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR20532-225,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IP, WB, IHC-P, Flow Cyt, mIHC, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProduct Specifications\u003cbr\u003e\nAnti-EpCAM antibody [EPR20532-225] (ab223582) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, ICC\/IF, IHC-P, IP, WB, mIHC in human samples.\u003cbr\u003e\nAnti-EpCAM antibody [EPR20532-225] (ab223582) specifically detects EpCAM (UniProt ID: P16422; Molecular weight: 33kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).\u003cbr\u003e\nQuality and Validation\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure Anti-EpCAM antibody [EPR20532-225] (ab223582) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nThe specificity of Anti-EpCAM antibody [EPR20532-225] (ab223582) has been confirmed by testing in knockout samples.\u003cbr\u003e\nAnti-EpCAM antibody [EPR20532-225] (ab223582) has been cited over 27 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nRelated Products\u003cbr\u003e\nConjugation-ready, carrier free format available for antibody clone EPR20532-225 -\u003cbr\u003e\nab225894\u003cbr\u003e\nAntibody clone EPR20532-225 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, PE, APC, Alexa Fluor® 594, Biotin, Alexa Fluor® 647 (\u003cbr\u003e\nab237395\u003cbr\u003e\nab237396\u003cbr\u003e\nab237397\u003cbr\u003e\nab237398\u003cbr\u003e\nab286811\u003cbr\u003e\nab314940\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.\u003cbr\u003e\nPathways\u003cbr\u003e\nEpCAM participates in regulatory mechanisms involving the Wnt\/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.\u003cbr\u003e\nEpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844035268777,"sku":"ab223582","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab223582","provider":"Iright","version":"1.0","type":"link"}