{"product_id":"abcam-ab223860","title":"Abcam, ab223860, Human Cardiac Troponin T ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Cardiac Troponin T ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cardiac Troponin T with a sensitivity of 68.2 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture media, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 68.2 pg\/mL,\u003cbr\u003e\nRange:195.3 - 12500 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Cardiac Troponin T ELISA Kit ab223860 is a rapid single-wash 90-min Sandwich ELISA to measure Human Cardiac Troponin T in cell culture media, serum. This SimpleStep sensitivity is 68.2 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman Cardiac Troponin T SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman Cardiac Troponin T ELISA Kit ab223860 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-Cardiac Troponin T antibody [EPR20266-143] - BSA and Azide free (Capture)\u003cbr\u003e\nab252655\u003cbr\u003e\n- Anti-Cardiac Troponin T antibody [EPR20266-143-6] - BSA and Azide free (Detector)\u003cbr\u003e\nab252655\u003cbr\u003e\nCardiac Troponin T is one of the three tropomyosin binding subunit that makes up the troponin complex with TnI and TnC being the other two subunits. The troponin complex is essential to the contraction of skeletal and heart muscles. Troponin T specifically binds to tropomyosin and helps position it on actin. Studies have shown elevated levels of troponin T after myocardial infarction and significantly associated with the incidence of heart failure and cardiovascular death.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843555283113,"sku":"ab223860","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab223860","provider":"Iright","version":"1.0","type":"link"}