{"product_id":"abcam-ab224828","title":"Abcam, ab224828, Anti-Cdk8 antibody","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRabbit Polyclonal CDK8 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CDK8 aa 400 to C-terminus.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human CDK8 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P49336\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab224828 was affinity purified using an epitope specific to Cdk8 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCdk8 also known as Cyclin-dependent kinase 8 forms an important component of the transcription regulation machinery. It has a molecular mass of about 66 kDa and is widely expressed in various tissues though its abundance can vary. Cdk8 operates largely in the nucleus of cells where it participates in regulating gene expression. Cdk8 acts as a kinase by modifying other proteins through phosphorylation which alters their activity and function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCdk8 plays a critical role in modulating transcriptional responses. It does this by becoming part of the Mediator complex which is essential for transcription regulation by RNA Polymerase II. The Mediator complex where Cdk8 acts as a kinase influences the initiation and regulation of gene expression. Cdk8 phosphorylates several transcription factors and other components affecting their ability to activate or repress transcription.\u003cbr\u003e\nPathways\u003cbr\u003e\nCyclin-dependent kinase 8 functions prominently in the Wnt\/β-catenin signaling and Notch signaling pathways both essential for development and cell differentiation. In the Wnt pathway it interacts closely with proteins like β-catenin to influence gene activation. In the Notch pathway it modulates transcription factors related to cellular differentiation processes. These interactions underline its role as a regulator in carefully managing cellular responses to signaling cues.\u003cbr\u003e\nCdk8 has been implicated in cancer and congenital disorders. Its overactivity can lead to uncontrolled cell growth contributing to tumor progression notably in colorectal cancer where it affects genes in the Wnt pathway. Beyond cancer its dysfunction links to congenital heart defects where it affects cardiogenesis-associated proteins such as NOTCH1. Cdk8’s involvement in these pathways highlights its part in both regular development and disease pathogenesis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46853988450473,"sku":"ab224828","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab224828","provider":"Iright","version":"1.0","type":"link"}