{"product_id":"abcam-ab22595","title":"Abcam, ab22595, Anti-Calnexin antibody - ER Marker","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-Calnexin antibody - ER Marker (ab22595) is a rabbit polyclonal antibody detecting Calnexin in  Western Blot, IP, ICC\/IF . Suitable for  Human, Mouse, Rat . - Over 480 publications - Trusted since 2006\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:IP, ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:Recognizes ER membrane, mitochondria and cis-Golgi\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-Calnexin antibody - ER Marker (ab22595) is a rabbit polyclonal antibody and is validated for use in ICC\/IF, IP, WB in human samples.\u003cbr\u003e\nAnti-Calnexin antibody - ER Marker (ab22595) has been cited over 482 times in peer reviewed journals, has been used for calnexin staining and is trusted by the scientific community.\u003cbr\u003e\nAbcam's high quality validation processes ensure Anti-Calnexin antibody - ER Marker (ab22595) has high sensitivity and specificity.\u003cbr\u003e\nThe specificity of Anti-Calnexin antibody - ER Marker (ab22595) has been confirmed by testing in knockout samples.\u003cbr\u003e\nAnti-Calnexin antibody - ER Marker (ab22595) has 28 independent reviews from customers.\u003cbr\u003e\nAnti-Calnexin antibody - ER Marker (ab22595) specifically detects Calnexin (UniProt ID: P27824; Molecular weight: 65kDa) and is sold in 100 µg selling sizes.\u003cbr\u003e\nCalnexin, an endoplasmic reticulum (ER) chaperone protein, plays a crucial role in oncology by influencing the immune response to tumors and cancer cell behavior. It can impair antitumor immunity by inhibiting T-cell infiltration and enhancing PD-1 expression on T cells, which suppresses their activity. Additionally, aberrant O-glycosylation of calnexin is linked to increased cancer cell migration and adhesion.\u003cbr\u003e\nExplore our\u003cbr\u003e\nrange of organelle-specific antibodies and dyes\u003cbr\u003e\nand discover why our reagents are deeply trusted by cell biologists.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle, Store in the dark\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCalnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCalnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.\u003cbr\u003e\nPathways\u003cbr\u003e\nCalnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.\u003cbr\u003e\nCalnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844141207721,"sku":"ab22595","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab22595","provider":"Iright","version":"1.0","type":"link"}