{"product_id":"abcam-ab226272","title":"Abcam, ab226272, Recombinant E. coli Beta-lactamase CTX-M-1 protein (His tag)","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRecombinant E. coli Beta-lactamase CTX-M-1 protein (His tag) is a Escherichia coli Full Length protein, in the 29 to 291 aa range, expressed in Escherichia coli, with \u0026gt;90%, suitable for SDS-PAGE, Mass Spec.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;90% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His tag N-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:P28585,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Escherichia coli,\u003cbr\u003e\nStorage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe CTX-M-1 beta-lactamase also known as CTX-M or CTX-M protein is a member of the class A beta-lactamases specifically extended spectrum beta-lactamases (ESBLs). This enzyme has a molecular weight of approximately 29 kDa. CTX-M-1 is primarily expressed in gram-negative bacteria particularly Escherichia coli frequently referred to as E. coli beta lactamase. Its primary function is to hydrolyze beta-lactam antibiotics including penicillins and cephalosporins rendering them inactive.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe CTX-M-1 enzyme plays an important role in antibiotic resistance. It hydrolyzes the beta-lactam ring structure of antibiotics which allows bacteria to survive in environments with these antimicrobials. CTX-M-1 functions as a single protein unit and does not form part of a larger complex. This resistance mechanism contributes to the increased survival of E. coli and other bacteria in clinical settings where antibiotics are often used.\u003cbr\u003e\nPathways\u003cbr\u003e\nCTX-M-1 beta lactamase impacts the antibiotic resistance pathway. It directly contributes to the breakdown of beta-lactam antibiotics which is an important step in resistance pathways. CTX-M-1 often interacts with other antibiotic resistance proteins like TEM and SHV beta-lactamases which also hydrolyze related compounds. These interactions complicate treatment strategies as the proteins together enhance a bacteria's resistance profile.\u003cbr\u003e\nCTX-M-1 beta lactamase plays a significant role in urinary tract infections (UTIs) and sepsis caused by drug-resistant E. coli. These infections are challenging to treat due to the presence of CTX-M-1 rendering many frontline antibiotics ineffective. The enzyme's activity relates to other proteins such as Klebsiella pneumoniae carbapenemase (KPC) which shares a similar antibiotic resistance role contributing to multidrug-resistant infections.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843868053673,"sku":"ab226272","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab226272","provider":"Iright","version":"1.0","type":"link"}