{"product_id":"abcam-ab227392","title":"Abcam, ab227392, Recombinant human ADK protein (Active)","description":"\u003cp\u003eSize: 20µg\u003cbr\u003e\nRecombinant human ADK protein (Active) is a Human Fragment protein, in the 22 to 362 aa range, expressed in Escherichia coli, with \u0026gt;95%, suitable for SDS-PAGE, FuncS, Mass Spec.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His tag N-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, Mass Spec, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Specific activity is \u0026gt; 30 pmol\/min\/μg and is defined as the amount of enzyme that converts 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37°C in a couple system with PK and LDH.,\u003cbr\u003e\nAccession:P55263,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.29% Sodium chloride, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAdenosine kinase (ADK) also known by its alternative name ATP:adenosine 5'-phosphotransferase is an enzyme with a molecular mass of approximately 40 kDa. This enzyme catalyzes the transfer of a phosphate group from ATP to adenosine forming AMP. ADK is expressed in several tissues with significant presence in the liver brain and lungs. This distribution suggests its versatile role in organ-specific metabolism and regulatory processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme modulates cellular adenosine levels which influences various physiological functions such as sleep regulation and immune responses. ADK does not operate as part of a larger protein complex but acts independently to maintain intracellular energy balance by regulating the pools of adenosine and AMP. The enzyme activity affects purine metabolism important in diverse cellular activities.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe activity of adenosine kinase fits prominently into the purine metabolism and energy homeostasis pathways. Its function maintains the cellular concentrations of adenosine a nucleotide affecting cellular signaling pathways including the AMP-activated protein kinase (AMPK) pathway. ADK interacts with enzymes like adenosine deaminase which collectively orchestrate the purine salvage pathway—pivotal in nucleotide recycling.\u003cbr\u003e\nIrregularities in ADK function have implications in neurological disorders and liver diseases. Abnormal ADK activity links to epilepsy due to its role in controlling adenosine concentrations affecting neurotransmitter release. Liver diseases such as hepatic steatosis have shown associations with altered ADK function impacting energy balance. The interplay with proteins such as AMP-activated protein kinase indicates potential therapeutic targets in disorders where metabolic regulation is compromised.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843935817897,"sku":"ab227392","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab227392","provider":"Iright","version":"1.0","type":"link"}