{"product_id":"abcam-ab227635","title":"Abcam, ab227635, Anti-PAX5 antibody [SP34] - ChIP Grade","description":"\u003cp\u003eSize: 100µL \/ 500µL \/ 1mL\u003cbr\u003e\nAnti-PAX5 antibody [SP34] - ChIP Grade (ab227635) is a rabbit monoclonal antibody detecting PAX5 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ChIP . Suitable for  Human, Rat . - Biophysical QC for unrivalled batch-batch consistency\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:SP34,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Rat, Human,\u003cbr\u003e\nApplications:IHC-P, WB, ChIP, IP, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-PAX5 antibody [SP34] - ChIP Grade (ab227635) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), ChIP in Human, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of PAX5?\u003cbr\u003e\nAnti-PAX5 [SP34] - ChIP Grade (ab227635) specifically detects a band for PAX5 (UniProt: Q02548) at a molecular weight of 42kDa.\u003cbr\u003e\nCollaboration\u003cbr\u003e\nAnti-PAX5 [SP34] - ChIP Grade (ab227635) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [SP34] also available for your convenience: ab227635, Carrier free -\u003cbr\u003e\nab237773\u003cbr\u003e\n, ab279606, APC -\u003cbr\u003e\nab310843\u003cbr\u003e\n, PE -\u003cbr\u003e\nab310914\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab310945\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab311063\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab311648\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab312921\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab313133\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321430\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A\/G, Storage buffer-pH: 7.6Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPAX5 also called B-cell-specific activator protein (BSAP) plays an important role in regulating gene expression during B-cell development. PAX5 is a transcription factor with an approximate mass of 50-55 kDa. It expresses mainly in B cells and in certain regions of the brain and testis. This protein binds DNA and controls the transcription of genes necessary for B-cell commitment and identity. Through processes like alternative splicing PAX5 generates multiple isoforms that contribute to various functional roles in its expressed locations.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPAX5 regulates the differentiation and maintenance of B-lymphocytes. It controls various genes critical for B-cell lineage commitment while repressing those involved in other lineages like T-cells and myeloid cells. PAX5 is part of a larger transcriptional complex interacting with other transcription factors to perform its function. During B-cell maturation PAX5 activates a specific gene set that helps shape the B-cell receptor repertoire a process important for effective immune responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nPAX5 integrates into key pathways such as the B-cell receptor signaling and JAK-STAT pathways. These pathways regulate processes like cell proliferation differentiation and apoptosis. In the B-cell receptor signaling PAX5 works alongside proteins like CD19 and BTK facilitating the transmission of activation signals necessary for B-cell survival. Connections in the JAK-STAT pathway involve interactions that promote gene expression changes necessary for B-cell functional activity further modulating the immune system's readiness.\u003cbr\u003e\nPAX5 is significantly associated with B-cell acute lymphoblastic leukemia (B-ALL) and non-Hodgkin lymphoma. In B-ALL mutations or deletions in PAX5 often lead to impaired B-cell development and uncontrolled cell proliferation. PAX5 mutations influence proteins like EBF1 and IL7R which are pivotal in B-cell progenitor activity and leukemogenesis. abnormalities in PAX5 expression or function can disrupt normal immune function leading to B-cell associated cancers and impairing immune surveillance highlighting its importance in maintaining immune system health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848520421545,"sku":"ab227635","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab227635","provider":"Iright","version":"1.0","type":"link"}