{"product_id":"abcam-ab227978","title":"Abcam, ab227978, Anti-ENO1 antibody [EPR19758]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-ENO1 antibody [EPR19758] (ab227978) is a rabbit monoclonal antibody detecting ENO1 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF . Suitable for  Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR19758,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), WB, IHC-P, ICC\/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:ab227978 does not cross-react with ENO2 or ENO3.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-ENO1 antibody [EPR19758] (ab227978) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of ENO1?\u003cbr\u003e\nAnti-ENO1 [EPR19758] (ab227978) specifically detects a band for ENO1 (UniProt: P06733) at a molecular weight of 47kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-ENO1 [EPR19758] (ab227978) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR19758] also available for your convenience: ab227978, Carrier free -\u003cbr\u003e\nab229378\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab303582\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab303584\u003cbr\u003e\n, PE -\u003cbr\u003e\nab305880\u003cbr\u003e\n, APC -\u003cbr\u003e\nab305881\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab305882\u003cbr\u003e\n, Alkaline Phosphatase -\u003cbr\u003e\nab308642\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab310344\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab311866\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab312335\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321462\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEnolase 1 or alpha-enolase is an important enzyme in the glycolytic pathway. It catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. We also refer to it as an enolase reaction. ENO1 protein has a molecular mass of approximately 47 kDa and is expressed in most tissues but found most abundantly in the brain muscle and liver. Additionally ENO1 can exist in both cytosolic and plasma membrane-associated forms playing functional roles in different cellular contexts.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe ENO1 protein serves multiple functions beyond its glycolytic activity. It also acts as a plasminogen receptor on the cell surface aiding in the modulation of pericellular and extracellular matrix degradation processes. Through this role ENO1 influences cell migration and tissue remodeling. This protein can form part of larger protein complexes contributing to its versatility in various cellular functions.\u003cbr\u003e\nPathways\u003cbr\u003e\nENO1 plays a critical role in glycolysis a central metabolic pathway that converts glucose into pyruvate generating ATP as a result. In this pathway ENO1 closely interacts with other enzymes like phosphoglycerate kinase and pyruvate kinase. Additionally as a plasminogen receptor ENO1 links to the plasminogen activation pathway affecting fibrinolysis and cellular response to tissue injury.\u003cbr\u003e\nENO1 is associated with cancer and neurodegenerative diseases. Overexpression or aberrant regulation of ENO1 often correlates with tumor progression and metastasis in cancers such as lung and breast cancer. In neurodegenerative disorders like Alzheimer's disease abnormal functioning of ENO1 can impact brain energy metabolism and neuron degeneration. ENO1's interaction with other proteins such as beta-enolase further connects it to these conditions influencing disease mechanisms and patient outcomes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848394920105,"sku":"ab227978","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab227978","provider":"Iright","version":"1.0","type":"link"}