{"product_id":"abcam-ab228549","title":"Abcam, ab228549, DAPI Staining Solution","description":"\u003cp\u003eSize: 2mL\u003cbr\u003e\nDAPI Staining Solution (ab228549) is a ready-to-use solution for labeling DNA in fluorescence microscopy. - Stains both live cells and fixed cells: DAPI passes through an intact cell membrane - Can also be used to detect mycoplasma or virus DNA in cell cultures - Cited in over 180 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay Platform:Fluorescence microscope\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nDAPI's absorption maximum when bound to double-stranded DNA is at 358 nm and its emission maximum is at 461 nm. Although is not as strongly fluorescent as when bound to DNA, it also binds to RNA, shifting its emission to around 500 nm.\u003cbr\u003e\nMolecular Weight: 350.25\u003cbr\u003e\nPurity: ≥95% by HPLC\u003cbr\u003e\nDAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like FITC and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red.\u003cbr\u003e\nBesides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures.\u003cbr\u003e\nDAPI Staining Solution Protocol Summary\u003cbr\u003e\n- Pellet cells by centrifugation\u003cbr\u003e\n- Resuspend cells in buffered salt solutions or media\u003cbr\u003e\n- Add DAPI stain\u003cbr\u003e\n- Incubate the sample for 15 to 60 minutes\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843538178217,"sku":"ab228549","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab228549","provider":"Iright","version":"1.0","type":"link"}