{"product_id":"abcam-ab229401","title":"Abcam, ab229401, Human Oncostatin M\/OSM ELISA Kit, Fluorescent","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Oncostatin M\/OSM ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Oncostatin M\/OSM with a sensitivity of 1.2 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Fluorescent Sandwich ELISA - 530\/570\/590 nm readout : works on any standard plate reader\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 1.2 pg\/mL,\u003cbr\u003e\nRange:2 - 2000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nOncostatin M\u003cbr\u003e\nin vitro\u003cbr\u003e\nCatchPoint\u003cbr\u003e\nSimpleStep ELISA\u003cbr\u003e\n(Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Oncostatin M protein in human serum, plasma, and cell culture supernatants.\u003cbr\u003e\nThis CatchPoint SimpleStep ELISA kit has been\u003cbr\u003e\noptimized for Molecular Devices Microplate Readers\u003cbr\u003e\n. Click\u003cbr\u003e\nfor a list of recommended Microplate Readers.\u003cbr\u003e\nIf using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at\u003cbr\u003e\nwww.softmaxpro.org\u003cbr\u003e\nThe CatchPoint\u003cbr\u003e\nSimpleStep ELISA\u003cbr\u003e\nemploys an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody\/analyte\/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint\u003cbr\u003e\nHRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530\/570\/590 nm Excitation\/Cutoff\/Emission.\u003cbr\u003e\nOncostatin M (OSM) is a 28-kDa pleiotropic cytokine of the IL-6 family that is a product of activated T lymphocytes, monocytes, neutrophils, and some tumor cells including breast cancer epithelial cells. Oncostatin M participates in a number of developmental, skeletal and immunological processes. Oncostatin M inhibits the proliferation of a number of tumor cell lines. It stimulates proliferation of AIDS-KS cells. Oncostatin M regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. It uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Oncostatin M is involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nOncostatin M (OSM) also known as Oncostatin M protein or OSM protein is a cytokine with significant roles in inflammation and hematopoiesis. This protein weighs around 28 kDa and is expressed mainly in activated T cells monocytes and macrophages. It interacts with specific receptors on the cell surface to transmit signals that regulate cell growth and function. Oncostatin M is part of the interleukin-6 (IL-6) family contributing to various cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nOncostatin M modulates a range of cellular activities including cell proliferation differentiation and apoptosis. This cytokine affects diverse cell types such as fibroblasts endothelial cells and epithelial cells showing particular influence in processes like tissue remodeling and immune response. It acts as a monomer and does not need assembly into a larger complex to exert its function. Its ability to trigger gene expression differentiates it from other closely related cytokines.\u003cbr\u003e\nPathways\u003cbr\u003e\nOncostatin M involves itself in significant pathways like the JAK\/STAT signaling pathway and the MAPK pathway. Through these pathways it promotes signal transduction essential for immune regulation and cellular response to external stimuli. OSM interacts with proteins like the gp130 receptor a common signaling component for cytokine receptors in its family and influences downstream effectors involved in these pathways.\u003cbr\u003e\nOncostatin M associates itself with inflammatory diseases such as rheumatoid arthritis and cardiovascular disorders. Its elevated levels in pathological conditions highlight its role in exacerbating inflammatory responses. In rheumatoid arthritis OSM promotes synovial inflammation while in cardiovascular diseases it may influence vascular remodeling. Connections to inflammatory proteins such as IL-6 further highlight its part in driving disease pathogenesis making it an intriguing target for therapeutic intervention.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843587461289,"sku":"ab229401","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab229401","provider":"Iright","version":"1.0","type":"link"}