{"product_id":"abcam-ab229421","title":"Abcam, ab229421, Mouse Leptin ELISA Kit, Fluorescent","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse Leptin ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Mouse Leptin with a sensitivity of 21.4 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Fluorescent Sandwich ELISA - 530\/570\/590 nm readout : works on any standard plate reader\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 21.4 pg\/mL,\u003cbr\u003e\nRange:23.4 - 48000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nLeptin\u003cbr\u003e\nin vitro\u003cbr\u003e\nCatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Leptin protein in mouse serum, plasma (citrate), urine and cell culture supernatants.\u003cbr\u003e\nThis CatchPoint SimpleStep ELISA kit has been\u003cbr\u003e\noptimized for Molecular Devices Microplate Readers\u003cbr\u003e\n. Click\u003cbr\u003e\nfor a list of recommended Microplate Readers.\u003cbr\u003e\nIf using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at\u003cbr\u003e\nwww.softmaxpro.org\u003cbr\u003e\nThe CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody\/analyte\/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530\/570\/590 nm Excitation\/Cutoff\/Emission.\u003cbr\u003e\nLeptin is a secreted protein factor produced by adipocytes. It regulates energy balance and body fat deposits. Leptin deficiency in humans and mice can cause obesity. Circulating levels of Leptin are regulated by food intake, insulin levels and pregnancy status. Mouse leptin has 96% and 85% protein sequence identity to rat and human leptin, respectively.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLeptin also known as the OB protein is a hormone with a molecular weight of approximately 16 kDa. Primarily it is produced by adipocytes in white adipose tissue and to a lesser extent in the placenta stomach and other tissues. This protein plays a significant role in energy homeostasis by conveying information to the central nervous system regarding fat storage. In scientific studies leptin is often measured using methods like the leptin ELISA kit or mouse leptin assay to monitor its levels and function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nLeptin regulates food intake and energy expenditure by binding to its receptors in the hypothalamus. It is an important part of the feedback loop to manage body weight and appetite. Leptin is not part of a larger protein complex and acts directly on its target tissues. By signal transduction pathways it influences cellular processes such as metabolism and even impacts fertility and immune responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nLeptin is involved in the neuroendocrine signaling pathway and the MAPK\/ERK pathway. In the neuroendocrine system leptin interacts with proteins like proopiomelanocortin (POMC) and neuropeptide Y (NPY) to manage appetite and energy use. Additionally it activates the JAK\/STAT pathway when binding to its receptor which influences gene expression involved in metabolism and inflammation.\u003cbr\u003e\nLeptin is linked to obesity and type 2 diabetes. Leptin deficiencies or resistance can lead to metabolic disorders as the hormone's signaling is disrupted affecting energy balance and storage. Its connection to adiponectin another hormone derived from fat tissue is critical in understanding these conditions; while leptin promotes inflammation adiponectin has anti-inflammatory effects. Exploring these proteins' roles and their balance offers insights into treatments for obesity-related disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855663517865,"sku":"ab229421","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab229421","provider":"Iright","version":"1.0","type":"link"}