{"product_id":"abcam-ab232477","title":"Abcam, ab232477, Anti-HIRA\/HIR antibody [EPR7416] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal HIRA\/HIR antibody. Carrier free. Suitable for IP, WB, ICC\/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR7416,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IF, WB, IP, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab232477 is the carrier-free version of\u003cbr\u003e\nab129169\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHIRA also known as HIR or HIR\/HIRA is a histone chaperone protein that plays an essential role in chromatin remodeling and nucleosome assembly. This protein has a mass of approximately 121 kDa. It is expressed in various tissues with notable presence in the liver heart and skeletal muscle. The HIRA protein interacts with other proteins to regulate the deposition of histone H3.3 which is important for processes like DNA replication and repair.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHIRA participates in the formation of a complex known as the HIRA-ASF1A-CABIN1 protein complex. This complex operates in chromatin assembly and modulates the incorporation of histone variant H3.3 affecting gene silencing and transcription regulation. Interacting with histone proteins HIRA serves an important function in genome stability and epigenetic mark establishment.\u003cbr\u003e\nPathways\u003cbr\u003e\nHIRA influences the histone replacement pathway significantly impacting gene expression and chromatin organization. HIRA works closely with ASF1A and CABIN1 proteins within this pathway. Additionally in the senescence-associated secretory phenotype pathway HIRA contributes to cellular aging processes by regulating transcriptional outcomes. The modulation of these pathways by HIRA exemplifies its key role in maintaining cellular functions.\u003cbr\u003e\nDefects in HIRA associate with hypertrophic cardiomyopathy and some cancer types such as breast cancer. HIRA alterations can disrupt chromatin dynamics potentially linking it with poor prognostic factors in these diseases. In particular the HIRA gene can interact with other genes involved in cardiomyopathies like MYH7 affecting heart muscle function. Through its role in chromatin remodeling HIRA connects to various pathologies where gene regulation and DNA damage responses are compromised.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46860390432937,"sku":"ab232477","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab232477","provider":"Iright","version":"1.0","type":"link"}