{"product_id":"abcam-ab232495","title":"Abcam, ab232495, Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal PFKM antibody. Carrier free. Suitable for WB, ICC\/IF, Flow Cyt (Intra) and reacts with Recombinant full length protein - Human, Mouse, Rat, Pig, Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR10734(B),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human, Pig,\u003cbr\u003e\nApplications:WB, Flow Cyt (Intra), ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab232495 is the carrier-free version of\u003cbr\u003e\nab154804\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPFKM also known as phosphofructokinase muscle type is a protein involved in glycolysis. It functions as an enzyme that catalyzes the phosphorylation of fructose 6-phosphate to fructose 16-bisphosphate. This reaction is important for the regulation of glucose metabolism. The PFKM protein has an approximate mass of 85 kDa and is mainly expressed in muscle tissues. Alternate names for PFKM include 6-phosphofructokinase type M and PFK-1 highlighting its critical role in glycolytic processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPFKM acts as an important regulatory component in glycolysis because it controls the rate-limiting step of this metabolic pathway. It forms a homo-tetramer complex which can vary in its exact subunit composition to modulate activity based on cellular energy requirements. The presence of allosteric sites on PFKM allows it to respond to different metabolic signals such as ATP and AMP levels highlighting its role in energy homeostasis. The ability to alter its conformation and enzymatic activity enables PFKM to efficiently regulate energy production and consumption at the cellular level.\u003cbr\u003e\nPathways\u003cbr\u003e\nPFKM plays a pivotal role in the glycolytic pathway facilitating the breakdown of glucose for energy production. This enzyme not only relates to the glycolysis pathway but also interacts with other key proteins such as hexokinase and pyruvate kinase forming a tightly controlled network for efficient metabolic flow. PFKM's activity influences downstream processes including the citric acid cycle and oxidative phosphorylation through its provision of pyruvate.\u003cbr\u003e\nDefects in PFKM are associated with glycogen storage disease type VII also known as Tarui's disease. This disorder results from a deficiency in PFKM activity leading to the accumulation of glycogen in muscle tissues and resulting in exercise intolerance. Additionally PFKM dysfunction has connections to cancer given that many cancer cells exhibit increased glycolytic activity. Considering its role in disease studying PFKM can provide insights into potential therapeutic targets for conditions characterized by metabolic abnormalities.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46860400951465,"sku":"ab232495","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab232495","provider":"Iright","version":"1.0","type":"link"}