{"product_id":"abcam-ab233621","title":"Abcam, ab233621, Mouse GFAP ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse GFAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse GFAP with a sensitivity of 8.7 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 5 citations\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Extracts,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 8.7 pg\/mL,\u003cbr\u003e\nRange:125 - 8000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse GFAP ELISA Kit ab233621 is a rapid single-wash 90-min ELISA kit to measure Mouse GFAP in tissue extracts. This SimpleStep ELISA® sensitivity is 8.7 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nMouse GFAP SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit recognizes both native and recombinant mouse GFAP protein in tissue extract samples only.\u003cbr\u003e\nMouse GFAP ELISA Kit ab233621 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nHow other researchers are using Mouse GFAP ELISA Kit ab233621\u003cbr\u003e\nMouse GFAP ELISA Kit ab233621 has been used to develop a nanosensor to identify mild traumatic brain injury\u003cbr\u003e\n, to test resolvin D2 as a therapy for neuropathic pain and bone cancer pain\u003cbr\u003e\n, and to study clearance of microglial debris for CNS homeostasis\u003cbr\u003e\nReferences:\u003cbr\u003e\nEster J Kwon et al. 2023, PMID: 37873031,\u003cbr\u003e\nXiaopeng Wang et al. 2023, PMID: 36672133,\u003cbr\u003e\nBo Peng, et al. 2022, PMID: 36280666\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation\u003cbr\u003e\n- Human\/Mouse GFAP Antibody Pair - BSA and Azide free\u003cbr\u003e\nab244094\u003cbr\u003e\n- Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture)\u003cbr\u003e\nab242692\u003cbr\u003e\n- Anti-GFAP antibody [EPR19996] - BSA and Azide free (Detector)\u003cbr\u003e\nab242937\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nMouse GFAP ELISA Kit ab233621 is commonly used to measure neuroinflammatory diseases such as Alzheimer's Disease and traumatic brain injury.\u003cbr\u003e\nOther ELISA kits to measure neuroinflammation include:\u003cbr\u003e\n- Mouse IL-6 ELISA kit\u003cbr\u003e\nab222503\u003cbr\u003e\n- Mouse TNF alpha ELISA kit\u003cbr\u003e\nab208348\u003cbr\u003e\n- Mouse IL-1 beta ELISA Kit\u003cbr\u003e\nab197742\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843551973545,"sku":"ab233621","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab233621","provider":"Iright","version":"1.0","type":"link"}