{"product_id":"abcam-ab234040","title":"Abcam, ab234040, Pyrophosphate Assay Kit (Colorimetric \/ Fluorometric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nPyrophosphate Assay Kit (Colorimetric \/ Fluorometric) (ab234040) uses an enzymatic method with one 30-60 minute incubation step to quantify free inorganic pyrophosphate (PPi) levels. Readout on any colorimetric (570 nm) or fluorometric (535\/587 nm) plate reader.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric\/Fluorometric,\u003cbr\u003e\nSample types:Tissue Lysate, Plasma, Cell culture extracts, Serum, Other biological fluids,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPyrophosphate assay kit ab234040 is used to quantify Pyrophosphate (abbreviated as PPi). Pyrophosphate, also called PPi and diphosphate, consists of two phosphate groups connected to each other through an oxygen atom.\u003cbr\u003e\nPyrophosphate assay principle\u003cbr\u003e\nIn the pyrophosphate assay, Pyruvate phosphate dikinase acts on pyrophosphate, in the presence of AMP and phosphoenolpyruvate, to produce pyruvate. Pyruvate is then acted on by pyruvate oxidase producing hydrogen peroxide as a product. The hydrogen peroxide reacts with a probe via a peroxidase to generate color (spectrophotometry at λ= 570 nm) and fluorescence (Ex\/Em = 535\/587 nm). Readout is on a colorimetric or fluorometric plate reader.\u003cbr\u003e\nMonomeric inorganic phosphate (Pi) does not interfere with the assay.\u003cbr\u003e\nPyrophosphate assay protocol summary\u003cbr\u003e\n- add samples, controls and standards to wells\u003cbr\u003e\n- add reaction mix and incubate for 30-60 mins at 37°C\u003cbr\u003e\n- analyse with a microplate reader\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nThis kit ab234040 is our most popular pyrophosphate assay kit. Pyrophosphate Assay Kit\u003cbr\u003e\nab112155\u003cbr\u003e\nuses an alternative method for pyrophosphate detection based on a fluorescent sensor.\u003cbr\u003e\nFor phosphate detection, our most popular product is phosphate assay\u003cbr\u003e\nab65622\u003cbr\u003e\nThis product was previously called K568 Biovision Pyrophosphate (PPi) Assay Kit (Fluorometric\/Colorimetric). Abcam acquired Biovision in 2021.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nInorganic pyrophosphate often abbreviated as PPi acts as a byproduct of several biosynthetic reactions. It possesses a molecular mass of 177.98 g\/mol. Pyrophosphate functions as an energy-rich intermediate that participates actively in reactions like the synthesis of nucleic acids and proteins. Inorganic pyrophosphatase an enzyme frequently associated with pyrophosphate metabolism catalyzes the hydrolysis of PPi releasing inorganic phosphate. This hydrolysis occurs release energy facilitating various biochemical processes. Pyrophosphate molecules are detected in tissues with high cell proliferation such as liver kidney and bones.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nInorganic pyrophosphate plays a critical part in bone mineralization and the regulation of calcium and phosphate metabolism. It influences the bone matrix's deposition by calcifying or inhibiting mineralization depending on the balance with phosphates. Within the extracellular matrix pyrophosphate remains associated with proteins such as alkaline phosphatase which converts PPi to phosphate promoting mineralization. Pyrophosphate's activity shows strong relevance in maintaining extracellular fluid stability preventing excessive calcification.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of inorganic pyrophosphate occurs prominently in the regulation of the ATP metabolism pathway and cartilage formation. It is closely related to the enzyme family of pyrophosphatases which include both soluble inorganic pyrophosphatase and membrane-integrated soluble pyrophosphatases. These enzymes orchestrate converting pyrophosphate into orthophosphate ions feeding into the pathways that regulate energy homeostasis and biomineralization. Interaction with proteins like ENPP1 and TNAP highlight its role within the broader context of nucleic acid synthesis and tissue calcification.\u003cbr\u003e\nAbnormal levels of inorganic pyrophosphate link to skeletal disorders like osteoporosis and osteoarthritis. High pyrophosphate concentrations can lead to pathological calcification processes prominently affecting joint health and bone density. The enzyme ectonucleotide pyrophosphatase\/phosphodiesterase 1 (ENPP1) connects to pyrophosphate's regulation of these diseases where it influences extracellular pyrophosphate concentration and subsequently mineralization. Understanding pyrophosphate dynamics provides insights into the pathophysiology of metabolic bone diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855606927529,"sku":"ab234040","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab234040","provider":"Iright","version":"1.0","type":"link"}