{"product_id":"abcam-ab234053","title":"Abcam, ab234053, Phagocytosis Assay Kit (Green Zymosan)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nPhagocytosis Assay Kit (Green Zymosan) (ab234053) uses Zymosan particles, that are fluorescently labeled with a green dye, to detect and quantify in vitro phagocytosis by fluorescent microscope, fluorometric plate reader or flow cytometry. - Ex\/Em 490\/520 nm - Validated as macrophage phagocytosis assay. - includes labeled Zymosan particles and assay buffer. Also includes quenching solution for readout on microscope \/ plate reader.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay Platform:Microplate reader, Fluor. microscope, Flow cyt.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhagocytosis Assay Kit (Green Zymosan) (ab234053) uses pre-labeled Zymosan particles as a tool for rapid and accurate detection and quantification of\u003cbr\u003e\nin vitro\u003cbr\u003e\nphagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and\/or inhibitors of phagocytosis and Toll-like receptors ligands (TLR).\u003cbr\u003e\nZymosan phagocytosis assay protocol summary\u003cbr\u003e\n- add Green Zymosan reagent to cells, and incubate for 2-3 hours\u003cbr\u003e\n- harvest cells by centrifugation, remove supernatant, and resuspend cells in Assay Buffer; repeat wash step 3 times\u003cbr\u003e\n- for fluorescent microscope or fluorometric plate reader analysis, use Quenching Solution\u003cbr\u003e\n- analyze cells with flow cytometer, fluorescent microscope, or fluorometric plate reader\u003cbr\u003e\nRelated phagocytosis assays\u003cbr\u003e\nPhagocytosis Assay Kit (Green E.coli)\u003cbr\u003e\nab235900\u003cbr\u003e\nPhagocytosis Assay Kit (Red E. coli)\u003cbr\u003e\nab235901\u003cbr\u003e\nPhagocytosis Assay Kit (Red Zymosan)\u003cbr\u003e\nab234054\u003cbr\u003e\nPhagocytosis Assay Kit (Zymosan, Colorimetric)\u003cbr\u003e\nab211156\u003cbr\u003e\nOther notes\u003cbr\u003e\nThis product was previously called K397 Biovision EZCell™ Phagocytosis Assay Kit (Green Zymosan). Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nPhagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. Zymosan prepared from yeast cell wall (Saccharomyces cerevisiae), and consisting of protein-carbohydrate complexes is frequently used as a pathogen in phagocytosis assays.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843621933225,"sku":"ab234053","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab234053","provider":"Iright","version":"1.0","type":"link"}