{"product_id":"abcam-ab234565","title":"Abcam, ab234565, Human FABP4 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman FABP4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FABP4 with a sensitivity of 2.7 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 2.7 pg\/mL,\u003cbr\u003e\nRange:4.69 - 300 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman FABP4 ELISA Kit (ab234565) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FABP4 protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FABP4 with 2.7 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n- Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n- High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n- Fully validated in biological samples\u003cbr\u003e\n- 96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nFatty acid-binding protein 4 (FABP4) also known as aP2 is a member of the fatty acid-binding protein family. It predominantly exists in adipocytes and macrophages serving an important role in lipid metabolism and transport. FABP4 facilitates the binding and transport of long-chain fatty acids and other hydrophobic molecules within cells. The molecular weight of FABP4 is approximately 14-15 kDa. It exhibits high expression levels in adipose tissue and is inducible in macrophages under certain conditions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein participates in the regulation of lipid homeostasis and energy metabolism. FABP4 is part of the lipid-binding protein family that helps maintain fatty acid equilibrium within cells. By controlling the transport and signaling of lipids FABP4 impacts lipid storage insulin sensitivity and inflammation processes. Its activity is not confined to a specific multimeric complex yet it interacts with other proteins to exert its functions contributing to significant metabolic pathways in the body.\u003cbr\u003e\nPathways\u003cbr\u003e\nFABP4 plays essential roles in the regulation of metabolic and inflammatory signaling pathways. It is intricately involved in the peroxisome proliferator-activated receptor gamma (PPARγ) pathway where it modulates adipose tissue development and glucose metabolism. In addition FABP4 has connections with the c-Jun N-terminal kinase (JNK) signaling pathway influencing stress and inflammatory responses. Interactions with these pathways place FABP4 at a link with proteins like PPARγ and JNK essential for cellular metabolic balance and responses to metabolic stress.\u003cbr\u003e\nResearchers connect FABP4 to conditions such as obesity and type 2 diabetes. Its expression levels correlate with insulin resistance and metabolic syndrome making it a target of interest in these metabolic disorders. FABP4 interacts with other proteins like adiponectin which is important in modulating glucose levels and fatty acid breakdown. Understanding FABP4's role in these pathways can facilitate the development of therapeutic strategies aimed at targeting metabolic dysfunctions associated with obesity and diabetes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843580940457,"sku":"ab234565","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab234565","provider":"Iright","version":"1.0","type":"link"}