{"product_id":"abcam-ab234578","title":"Abcam, ab234578, Human ALT ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests \/ 10 x 96Tests\u003cbr\u003e\nHuman ALT ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human alanine aminotransferase (ALT) with a sensitivity of 47.34 pg\/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA with a 450 nm readout; compatible with any standard plate reader - Different formats for different needs, including 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready carrier-free antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture media, Citrate plasma, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 47.34 pg\/mL,\u003cbr\u003e\nRange:109.38 - 7000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman ALT ELISA Kit ab234578 is a rapid single-wash 90-min ELISA kit to measure Human ALT in . This SimpleStep ELISA® sensitivity is 47.34 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman ALT SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit recognizes both native and recombinant human ALT protein in serum, plasma, and cell culture supernatant samples only. Tissue extract samples have not been tested with this kit. This kit is incompatible with plasma- heparin and cell extract samples.\u003cbr\u003e\nHuman ALT ELISA Kit ab234578 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nHow other researchers are using Human ALT ELISA Kit ab234578\u003cbr\u003e\nHuman ALT ELISA Kit ab234578 has been used to validate a human Liver-Chip as a model for predicting drug-induced liver injury\u003cbr\u003e\n(1)(2)\u003cbr\u003e\nand to study novel therapeutics to hepatitis B\u003cbr\u003e\nReferences:\u003cbr\u003e\nDaniel Levner et al. 2022, PMID: 36473994,\u003cbr\u003e\nJaney Ronxhi et al. 2022, PMID: 35790095,\u003cbr\u003e\nSung-Gyoo Park, et al. 2019, PMID: 31097716\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Human ALT ELISA Kit only requires a maxmum of 12.5µL\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation\u003cbr\u003e\n- Human ALT Antibody Pair - BSA and Azide free\u003cbr\u003e\nab244121\u003cbr\u003e\n- Anti-Alanine Transaminase antibody [EPR21068-102] - BSA and Azide free (Capture)\u003cbr\u003e\nab244765\u003cbr\u003e\n- Anti-Alanine Transaminase antibody [EPR21068-86] - BSA and Azide free (Detector)\u003cbr\u003e\nab245036\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman ALT ELISA kit ab234578 is commonly used by researchers to measure liver function.\u003cbr\u003e\nOther ELISA kits to measure liver function include:\u003cbr\u003e\n- Human AST ELISA kit\u003cbr\u003e\nab263881\u003cbr\u003e\n- Human Albumin ELISA kit\u003cbr\u003e\nab179887\u003cbr\u003e\n- Albumin (BCG) Assay Kit (Colorimetric)\u003cbr\u003e\nab235628\u003cbr\u003e\nThese ELISA kits are also available for Mouse\/Rat:\u003cbr\u003e\n- Mouse ALT ELISA kit\u003cbr\u003e\nab282882\u003cbr\u003e\n- Mouse AST ELISA kit\u003cbr\u003e\nab263882\u003cbr\u003e\n- Mouse Albumin ELISA kit\u003cbr\u003e\nab207620\u003cbr\u003e\n- Rat ALT ELISA kit abab234579\u003cbr\u003e\n- Rat AST ELISA kit\u003cbr\u003e\nab263883\u003cbr\u003e\n- Rat Albumin ELISA kit\u003cbr\u003e\nab235642\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlanine transaminase also known as ALT or alanine aminotransferase is an important enzyme in the body. It has a molecular mass of approximately 54 kDa and catalyzes the conversion between alanine and pyruvate by transferring an amino group. ALT is mainly expressed in the liver but also present in other tissues like the heart and muscles. It is often measured as 'ALT activity' in clinical settings to ascertain liver health. In laboratories an 'ALT assay' or 'ALT kit' can determine alanine transaminase activity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAlanine transaminase plays a critical role in amino acid metabolism and energy production. It facilitates the transamination process which is significant for the balance of amino acids and carbohydrates. ALT as a standalone enzyme does not form part of a protein complex but works closely with alanine aminotransferase reactions. It helps maintain the cellular nitrogen balance linking nitrogen metabolism with energy pathways.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe role of alanine transaminase is to interconnect carbohydrate and amino acid metabolism. It participates in the gluconeogenesis pathway and the citric acid cycle important for energy production. Through its activity in these pathways it interacts with proteins like aspartate aminotransferase (AST) affecting the balance of amino and keto acids in cells.\u003cbr\u003e\nAlanine transaminase levels indicate liver conditions such as hepatitis and liver cirrhosis. Elevated ALT activity suggests liver damage or inflammation. It also connects to conditions involving muscular injury. In these scenarios increased presence and activity of AST often accompany ALT providing a broader picture of the extent of tissue damage. Understanding the involvement of ALT in these pathways helps diagnose and monitor the progression of relevant health issues.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843536933033,"sku":"ab234578","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab234578","provider":"Iright","version":"1.0","type":"link"}