{"product_id":"abcam-ab234982","title":"Abcam, ab234982, Anti-CYP7A1 antibody","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRabbit Polyclonal CYP7A1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CYP7A1 aa 300-450.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Recombinant Fragment Protein within Human CYP7A1 aa 300-450. The exact immunogen used to generate this antibody is proprietary information.P22680\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purity \u0026gt;95%., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCYP7A1 also known as cholesterol 7 alpha-hydroxylase plays an important role as an enzyme in the liver. It has a molecular mass of approximately 57 kDa. This enzyme catalyzes the rate-limiting step in the catabolism of cholesterol to bile acids by converting cholesterol to 7 alpha-hydroxycholesterol. The expression of CYP7A1 is mainly in the liver where its activity is important for maintaining cholesterol and bile acid homeostasis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCholesterol 7 alpha-hydroxylase plays an important role in the primary pathway for bile acid biosynthesis. It not only affects the breakdown of cholesterol but also influences lipid metabolism. Although it does not function as part of a larger protein complex CYP7A1 activity is a critical point of regulation within these metabolic processes. Its efficiency can affect plasma cholesterol levels hence impacting overall lipid homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nCYP7A1 is central to the classical pathway of bile acid synthesis. In this pathway it interacts directly with several other proteins including HMG-CoA reductase which is involved in cholesterol synthesis. Additionally CYP7A1 activity influences the regulatory axis of liver X receptors (LXR) which serve to manage cholesterol fatty acids and glucose homeostasis. The balance between these pathways determines cholesterol levels in the body and can modulate metabolic health.\u003cbr\u003e\nDysfunction or altered regulation of CYP7A1 contributes significantly to hypercholesterolemia and gallstone disease. Changes in CYP7A1 expression can result in imbalances in cholesterol levels leading to these conditions. The protein's activity is often compared to mitochondrial enzymes related to bile acid synthesis such as CYP27A1 connecting them both in the intricate network of cholesterol regulation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848594149545,"sku":"ab234982","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab234982","provider":"Iright","version":"1.0","type":"link"}