{"product_id":"abcam-ab236023","title":"Abcam, ab236023, Anti-Brachyury \/ Bry antibody [EPR18113] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Brachyury \/ Bry antibody. Carrier free. Suitable for IP, WB, ICC\/IF, IHC - Wmt, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR18113,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:IHC-P, IP, ICC\/IF, WB, Flow Cyt (Intra), IHC - WmtSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab236023 is the carrier-free version of\u003cbr\u003e\nab209665\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Brachyury protein also known as Bry or T acts as a transcription factor and belongs to the T-box family of genes. It consists of about 435 amino acids giving it an estimated mass of around 47 kDa. Brachyury plays a critical role in the development of the posterior mesoderm and nucleus pulposus during embryogenesis. It is strongly expressed in the notochord mesoderm and some tumor tissues. The protein's presence can be detected using specific assays such as Brachyury IHC (immunohistochemistry) and Brachyury stain which help identify Brachyury marker expression in both normal and pathological states.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe Brachyury protein influences the morphogenetic movement in mesoderm development. It directly interacts with specific DNA sequences to regulate the transcription of target genes. Brachyury often operates as part of a larger transcriptional complex that manages the differentiation and migration of cells during embryogenesis. The gene's expression indicates mesodermal differentiation and is important for cell fate decisions within developing embryos.\u003cbr\u003e\nPathways\u003cbr\u003e\nBrachyury plays a significant role in the Wnt signaling and the TGF-beta pathways. These pathways are essential in controlling cell proliferation and differentiation. Brachyury directly impacts the regulation of these pathways influencing other proteins like beta-catenin in Wnt signaling. This interconnectedness suggests its broader role in cellular communication and development processes potentially affecting gene expression across these pathways.\u003cbr\u003e\nBrachyury has been associated with various cancers such as chordoma and other related tumors. Chordoma specifically involves the notochord where Brachyury marks the disease's presence. Other proteins like E-cadherin interact with Brachyury in tumor progression and metastasis. Understanding Brachyury's function helps in diagnosing and potentially targeting treatment options for these ailments.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850858713257,"sku":"ab236023","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab236023","provider":"Iright","version":"1.0","type":"link"}