{"product_id":"abcam-ab236056","title":"Abcam, ab236056, Anti-UBE2M\/UBC12 antibody [EPR5333] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal UBE2M\/UBC12 antibody. Carrier free. Suitable for IHC-P, WB, ICC\/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR5333,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-P, Flow Cyt (Intra), ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab236056 is the carrier-free version of\u003cbr\u003e\nab109507\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nUBE2M also called UBC12 is an E2 enzyme involved in the process of neddylation which is a type of ubiquitin-like modification. The protein has a molecular mass of approximately 22 kDa. It activates and transfers NEDD8 a ubiquitin-like protein to specific substrates. UBE2M is expressed in various tissues with high expression levels in the liver muscle and kidney tissues. Its precise mechanical role is connecting to NEDD8 and collaborating with E3 ligases to modify substrates.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nE2 conjugating enzyme such as UBE2M functions in the regulation of the cell cycle signal transduction and other cellular processes. It is important in the neddylation pathway where it facilitates the covalent attachment of the NEDD8 molecule to Cullin proteins. UBE2M associates with the cullin-RING ligase complex assisting in the alteration of protein stability and function within cells. The proper operation of this enzyme is essential for maintaining cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nNeddylation modulates ubiquitination and acts as a regulator in protein degradation pathways. UBE2M occupies a central role in the neddylation pathway which intersects with the ubiquitin-proteasome system involving proteins like Cullin and NEDD8. Both neddylation and ubiquitination pathways share overlapping components and functions making UBE2M critical in selective protein degradation impacting processes such as DNA replication and repair and response to stress.\u003cbr\u003e\nThe incorrect function of UBE2M has links to cancer and neurodegenerative diseases. In cancer misregulation of the neddylation pathway can lead to unregulated cell growth and survival due to the aberrant degradation of key proteins. Abnormal UBE2M activity impacts the regulation of the tumor suppressor protein p53 through interaction with other neddylation-related proteins affecting cell cycle control. In neurodegenerative diseases alterations in protein homeostasis and aggregation occur related to the dysregulation of neddylation influencing proteins involved in neuronal function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46856050802857,"sku":"ab236056","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab236056","provider":"Iright","version":"1.0","type":"link"}