{"product_id":"abcam-ab237007","title":"Abcam, ab237007, Recombinant Human p53 protein","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRecombinant Human p53 protein is a Human Full Length protein in the 1 to 393 aa range with \u0026gt;90% purity and suitable for SDS-PAGE and mass spectrometry. The predicted molecular weight of ab237007 protein is 59.7 kDa. - Save time and ensure accurate results - use our p53 protein as a control\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;90% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:6x His tag N-Terminus SUMO tag N-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:P04637,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nEnsure the validity of your result using our recombinant human Cellular tumor antigen p53 (TP53) protein ab237007 as a positive control in SDS-PAGE and mass spectrometry.\u003cbr\u003e\nCheck out our protein gel staining guide for SDS-PAGE\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nP53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.\u003cbr\u003e\nPathways\u003cbr\u003e\nP53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.\u003cbr\u003e\nP53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843815231657,"sku":"ab237007","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab237007","provider":"Iright","version":"1.0","type":"link"}