{"product_id":"abcam-ab237712","title":"Abcam, ab237712, Anti-CTLA4 antibody [CAL49]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nAnti-CTLA4 antibody [CAL49] (ab237712) is a rabbit monoclonal antibody detecting CTLA4 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P . Suitable for  Human, Mouse, Rhesus monkey . - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:CAL49,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Human, Rhesus monkey,\u003cbr\u003e\nApplications:WB, Flow Cyt (Intra), IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-CTLA4 antibody [CAL49] (ab237712) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rhesus monkey samples.\u003cbr\u003e\nWhat is the molecular weight of CTLA4?\u003cbr\u003e\nAnti-CTLA4 [CAL49] (ab237712) specifically detects a band for CTLA4 (UniProt: P16410) at a molecular weight of 25kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-CTLA4 [CAL49] (ab237712) was first used in a scientific publication in 2018 and has been cited over 20 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-CTLA4 [CAL49] (ab237712) has over 5 independent reviews from customers.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10μl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [CAL49] also available for your convenience: ab237712, Carrier free -\u003cbr\u003e\nab251599\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab270127\u003cbr\u003e\n, PE -\u003cbr\u003e\nab270128\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab283489\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purity \u0026gt;99%., Storage buffer-pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCTLA-4 also known as CD152 is an immune checkpoint receptor with a molecular weight of approximately 34 kDa. This protein is expressed on the surface of T-cells especially after activation and sometimes on regulatory T-cells (Tregs). CTLA-4 competes with CD28 for binding to the same ligands CD80 and CD86 on antigen-presenting cells. Unlike CD28 which stimulates T-cell activation CTLA-4 sends inhibitory signals to downregulate immune responses. By doing this CTLA-4 helps maintain immune system homeostasis and prevents autoimmune reactions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe CTLA-4 protein functions as a critical regulator of the immune system. It interacts with CD80\/CD86 in a complex that effectively transmits inhibitory signals to T-cells. CTLA-4 controls the amplitude of the initial activation of T-cells ensuring that the body's immune responses are kept under control. In regulatory T-cells CTLA-4 engagement contributes to their immunosuppressive functions through which they maintain tolerance to self-antigens and prevent overactive immune reactions.\u003cbr\u003e\nPathways\u003cbr\u003e\nCTLA-4 involves itself in the adaptive immune response pathways specifically the regulation of T-cell activity. CTLA-4's interaction with CD80\/CD86 modulates pathways associated with TCR (T-cell receptor) signaling. When CTLA-4 binds its ligands it recruits phosphatases such as SHP-2 that dephosphorylate signaling proteins leading to the downregulation of T-cell interaction. This pathway interaction exemplifies how CTLA-4 acts in concert with proteins like CD28 to finely tune immune responses.\u003cbr\u003e\nCTLA-4 plays a role in autoimmune diseases and cancer. The dysregulation of CTLA-4 function can lead to autoimmune disorders where the immune system attacks the body's own tissue. In cancer tumor cells may manipulate CTLA-4 pathways to evade immune surveillance. Targeted therapies like anti-CTLA-4 antibodies such as ipilimumab have been developed to block CTLA-4 aiming to enhance the immune system's ability to attack cancer cells. These treatments highlight the role of CTLA-4 in balancing immune activity and the potential to alter this balance for therapeutic benefit.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844139143337,"sku":"ab237712","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab237712","provider":"Iright","version":"1.0","type":"link"}