{"product_id":"abcam-ab237726","title":"Abcam, ab237726, Anti-PD-L1 antibody [CAL10]","description":"\u003cp\u003eSize: 20µL \/ 50µL \/ 1mL\u003cbr\u003e\nAnti-PD-L1 antibody [CAL10] (ab237726) is a rabbit monoclonal antibody detecting PD-L1 in  Western Blot, IP, IHC-P, ICC\/IF, ELISA, mIHC . Suitable for  Human, . - KO validated for confirmed specificity - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:CAL10,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:mIHC, ELISA, IHC-P, IP, ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-PD-L1 antibody [CAL10] (ab237726) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF), ELISA, Multiplex IHC (mIHC) in Human, samples.\u003cbr\u003e\nWhat is the molecular weight of PD-L1?\u003cbr\u003e\nAnti-PD-L1 [CAL10] (ab237726) specifically detects a band for PD-L1 (UniProt: Q9NZQ7) at a molecular weight of 33kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-PD-L1 [CAL10] (ab237726) was first used in a scientific publication in 2018 and has been cited over 10 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10μl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-PD-L1 antibody [CAL10] (ab237726) has been confirmed by Western blot testing in CD274 Knockout A549 cell line,\u003cbr\u003e\nab267054\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [CAL10] also available for your convenience: ab237726, Carrier free -\u003cbr\u003e\nab251611\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab267565\u003cbr\u003e\nab279292\u003cbr\u003e\nab279293\u003cbr\u003e\nab279294\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab279304\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab279305\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab279306\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab307643\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purity \u0026gt;99%, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPD-L1 also known as Programmed Death-Ligand 1 or CD274 is a protein involved in immune modulation. Mechanically PD-L1 interacts with its receptors particularly PD-1 to regulate cellular immune responses. This transmembrane protein has a calculated molecular weight of approximately 33 kDa. PD-L1 is expressed on various cell types including tumor cells and immune cells such as dendritic cells macrophages and B cells. Its expression is often upregulated in response to inflammatory cytokines.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPD-L1 plays a central role in immune evasion mechanisms utilized by tumors. It is not part of a larger protein complex but directly interacts with PD-1 and CD80. When PD-L1 binds to PD-1 it sends inhibitory signals leading to decreased T cell activation and proliferation allowing cancer cells to avoid immune destruction. PD-L1 expression provides a mechanism for tumors to suppress immune surveillance facilitating tumor progression.\u003cbr\u003e\nPathways\u003cbr\u003e\nPD-L1 is integral to the immune checkpoint pathway which is an important regulator of immune response. The interaction between PD-L1 and PD-1 provides a mechanism for immune tolerance which is part of the broader adaptive immune system pathway. PD-L1 is related to other immune checkpoint proteins such as CTLA-4 in its function to limit autoreactivity and promote immune homeostasis.\u003cbr\u003e\nPD-L1 is most associated with cancer particularly in tumors such as melanoma and non-small cell lung cancer. PD-L1 expression on tumor cells often correlates with poor prognosis. PD-L1 directly interacts with PD-1 in these cancers a target for immunotherapies such as checkpoint inhibitors which aim to block this interaction and restore immune activity against tumors. PD-L1 involvement extends to autoimmune disorders where altered expression can impact tolerance and lead to immune-related tissue damage.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844049621161,"sku":"ab237726","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab237726","provider":"Iright","version":"1.0","type":"link"}