{"product_id":"abcam-ab238169","title":"Abcam, ab238169, Anti-IRG1 antibody [EPR22066] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal IRG1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR22066,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab238169 is the carrier-free version of\u003cbr\u003e\nab222411\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nIRG1 also known as Immune-Responsive Gene 1 protein has a molecular weight of approximately 50 kDa. This protein is expressed largely in immune cells such as macrophages and dendritic cells. Mechanically IRG1 catalyzes the production of itaconate from cis-aconitate during the citric acid cycle an action that has gained significant interest due to its immunomodulatory effects. The localization of IRG1 in immune cells suggests a role in the response to pathogenic threats and regulation of inflammation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nIRG1 is important for immune response regulation by producing itaconate an anti-inflammatory metabolite. Itaconate acts on innate immune signaling pathways to dampen excessive inflammatory responses. Though IRG1 is not part of a larger protein complex it interacts closely with cellular metabolism. This interaction plays a significant role in metabolic reprogramming during infections thereby influencing cytokine production and the activation state of cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nIRG1 plays a significant role in the tricarboxylic acid (TCA) cycle and is associated with the innate immune response. The protein shifts cellular metabolism towards cataplerosis to accommodate the need for itaconate production. IRG1 is connected to regulatory proteins such as the transcription factor NRF2 which mediates antioxidative responses. Itaconate a product of IRG1 activity can modulate pathways like the NF-kB signaling pathway attenuating inflammatory responses.\u003cbr\u003e\nIRG1 has been implicated in atherosclerosis and tuberculosis. In atherosclerosis IRG1 contributes to the anti-inflammatory state within macrophages which can alter plaque formation. Through itaconate IRG1 interacts with inflammasome components potentially impacting the pathology of the disease. In tuberculosis IRG1-induced itaconate production helps in macrophage defense against Mycobacterium tuberculosis. Additionally pathways involving IRG1 often intersect with proteins like HIF-1α under hypoxic conditions relevant to the progression and management of these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848564625577,"sku":"ab238169","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab238169","provider":"Iright","version":"1.0","type":"link"}