{"product_id":"abcam-ab238429","title":"Abcam, ab238429, Anti-Myosin light chain kinase\/MLCK antibody [EP1458Y] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Myosin light chain kinase\/MLCK antibody. Carrier free. Suitable for IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EP1458Y,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human, Mouse, Rat,\u003cbr\u003e\nApplications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab238429 is the carrier-free version of\u003cbr\u003e\nab76092\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMyosin light chain kinase also known as MLCK is an enzyme that phosphorylates myosin light chain proteins facilitating muscle contraction. This kinase primarily interacts with myosin a molecular motor protein responsible for converting chemical energy into mechanical force. The myosin light chains are a part of this complex and are important for regulating myosin's ATPase activity. The molecular weight of MLCK is approximately 130 kDa. It is expressed in various tissues including smooth muscle skeletal muscle and endothelial cells reflecting its central role in these muscle types.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMyosin light chain kinase plays a critical role in the regulation of muscle contraction and cellular motility. By phosphorylating the myosin light chain MLCK allows the interaction of actin and myosin which is essential for muscle contraction and movement. In smooth muscle MLCK activity is important for regulating vascular tone and blood pressure. As part of a complex with calmodulin MLCK activation depends on calcium levels linking its activity with intracellular signaling pathways influenced by calcium ions.\u003cbr\u003e\nPathways\u003cbr\u003e\nMyosin light chain kinase is an integral part of the calcium-calmodulin pathway where it acts in response to increased intracellular calcium levels signaled through various cellular activities. This kinase is also involved in the RhoA-Rho kinase pathway which regulates smooth muscle contraction and cytoskeletal dynamics. Related proteins in these pathways include calmodulin whose binding activates MLCK and RhoA a small GTPase associated with the assembly of actin-myosin filaments.\u003cbr\u003e\nMyosin light chain kinase has links to cardiovascular diseases such as hypertension due to its role in smooth muscle contraction and blood vessel function. Asthma is another condition related to MLCK as the kinase influences airway smooth muscle tone which can affect breathing. Dysregulation of MLCK activity can manifest in altered muscle contractility in these diseases making it a potential target for therapeutic intervention. Research continues to explore the kinase's interactions with calmodulin and other proteins within these disease pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848527630505,"sku":"ab238429","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab238429","provider":"Iright","version":"1.0","type":"link"}