{"product_id":"abcam-ab239400","title":"Abcam, ab239400, Anti-Integrin alpha 5 antibody [EPR7854] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Integrin alpha 5 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC\/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR7854,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), WB, IP, IHC-P, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab239400 is the carrier-free version of\u003cbr\u003e\nab150361\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nIntegrin alpha 5 also known as CD49e or ITGA5 is a subunit of the integrin family that forms heterodimers with beta 1 integrin. It has an approximate mass of 130 kDa. This protein mainly expresses in various tissues including fibroblasts endothelial cells and certain epithelial cells. Integrin alpha 5 mediates cell adhesion by binding to fibronectin an important extracellular matrix protein helping cells stay attached to their surrounding environment.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nIntegrin alpha 5 plays a significant role in cellular processes such as migration proliferation and differentiation. It pairs with beta 1 integrin to form the alpha 5 beta 1 integrin complex. This complex facilitates the transmission of mechanical and chemical signals between the extracellular matrix and the cell interior. Integrin alpha 5 also interacts with intracellular proteins like talin and kindlin which are essential for initiating and strengthening the linkage to the actin cytoskeleton.\u003cbr\u003e\nPathways\u003cbr\u003e\nIntegrin alpha 5 is involved in the regulation of several critical signaling pathways. It prominently features within the MAPK\/ERK pathway and the focal adhesion signaling pathway. Through these pathways integrin alpha 5 influences cell survival proliferation and migration. It interacts with proteins such as paxillin and focal adhesion kinase (FAK) within these pathways contributing to cellular responses to external stimuli.\u003cbr\u003e\nIntegrin alpha 5 is linked to conditions such as cancer and fibrosis. Overexpression or aberrant regulation of this integrin often associates with tumorigenesis particularly in breast and colorectal cancers enhancing cancer cell migration and invasion. Integrin alpha 5 is also connected to proteins like matrix metalloproteinases (MMPs) which play roles in extracellular matrix remodeling and are involved in fibrotic diseases. These associations highlight the importance of integrin alpha 5 in pathological processes and its potential as a target for therapeutic intervention.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851592454313,"sku":"ab239400","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab239400","provider":"Iright","version":"1.0","type":"link"}