{"product_id":"abcam-ab239709","title":"Abcam, ab239709, GSH+GSSG \/ GSH Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test \/ 2000Test\u003cbr\u003e\nGSH+GSSG \/ GSH Assay Kit (Colorimetric) ab239709 provides a convenient method to quantify either total glutathione, or the reduced form glutathione alone, using any colorimetric (412 nm) plate reader. - Cited in \u0026gt;40 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Serum, Cell culture extracts,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nGSH+GSSG \/ GSH Assay Kit (Colorimetric) (ab239709) provides a convenient, colorimetric method for analyzing either total glutathione or the reduced form glutathione alone using a microtiter plate reader.\u003cbr\u003e\nThe assay is based on the glutathione recycling system by DTNB and glutathione reductase. DTNB and glutathione (GSH) react to generate 2-nitro-5-thiobenzoic acid which has a yellow color. Therefore, GSH concentration can be determined by measuring absorbance at 412 nm.\u003cbr\u003e\nThe generated GSSG can be reduced back to GSH by glutathione reductase, and GSH reacts with DTNB again to produce more 2-nitro-5-thiobenzoic acid. Therefore, the recycling system dramatically improves the sensitivity of total glutathione detection.\u003cbr\u003e\nThe kit includes the 5-Sulfosalicylic acid (SSA) for the removal of proteins from samples and for the protection of GSH oxidation and g-glutamyl transpeptidase reaction.\u003cbr\u003e\nThe kit can quantify glutathione from 1-100 ng\/well in a 200 µL reaction. For detecting lower glutathione concentrations, such as in blood samples, increasing reaction time will generate stronger signal.\u003cbr\u003e\nThe kit can also specifically detect the reduced form of glutathione (GSH) by omitting the glutathione reductase from the reaction mixture. The sensitivity for detecting the reduced form of glutathione (without recycling system) is 100 times lower than detecting the total glutathione.\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K261 Glutathione Colorimetric Assay Kit. K261-100 is the same size as the 100 test size of ab239709.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843611152553,"sku":"ab239709","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab239709","provider":"Iright","version":"1.0","type":"link"}