{"product_id":"abcam-ab240028","title":"Abcam, ab240028, Anti-DDX5 antibody [EPR7239] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal DDX5 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC\/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR7239,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), ICC\/IF, WB, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab240028 is the carrier-free version of\u003cbr\u003e\nab126730\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDDX5 also known as DEAD-box helicase 5 or p68 is an RNA helicase important to various RNA processing tasks. With a molecular mass of approximately 68 kDa DDX5 unwinds RNA secondary structures using energy from ATP hydrolysis. It expresses widely in human tissues with higher levels observed in regions of active cellular proliferation. The expression pattern reflects its significant involvement in pathways that require dynamic RNA structure modifications.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe function of DDX5 facilitates RNA splicing transcription and ribosome biogenesis. DDX5 is a component of various RNA processing complexes interacting with other proteins to modify pre-mRNA and other RNA molecules. It plays a role in modulating gene expression and ensuring the proper processing of precursor RNA transcripts. This makes DDX5 important in cellular differentiation and development influencing stem cell renewal and pluripotency.\u003cbr\u003e\nPathways\u003cbr\u003e\nDDX5 participates in the Wnt\/β-catenin and p53 signaling pathways. In the Wnt\/β-catenin pathway DDX5 interacts with proteins such as β-catenin to influence gene transcription involved in cell growth and differentiation. Within the p53 pathway DDX5 modulates the activity of p53 a protein known for its role in DNA damage response and apoptosis. These interactions position DDX5 at the intersection of multiple regulatory networks that govern cellular proliferation and survival.\u003cbr\u003e\nAberrant DDX5 expression links to cancer and neurodegenerative diseases. Overexpression or dysregulation of DDX5 has been observed in various cancers including breast cancer where it can interact with proteins such as estrogen receptor alpha promoting tumor progression. In neurodegenerative disorders particularly amyotrophic lateral sclerosis (ALS) DDX5 forms toxic aggregates with TDP-43 a protein important in RNA binding and processing. Understanding DDX5's involvement in these diseases offers insight into potential therapeutic targets and diagnostic markers.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851608215721,"sku":"ab240028","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab240028","provider":"Iright","version":"1.0","type":"link"}