{"product_id":"abcam-ab240144","title":"Abcam, ab240144, Anti-MBD3 antibody [EPR9913] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal MBD3 antibody. Carrier free. Suitable for ChIP, WB, ICC\/IF, Flow Cyt (Intra), IP and reacts with Mouse, Human, Rat, Cow samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR9913,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Cow, Human,\u003cbr\u003e\nApplications:WB, Flow Cyt (Intra), ChIP, ICC\/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab240144 is the carrier-free version of\u003cbr\u003e\nab157464\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe MBD3 protein also known as Methyl-CpG-binding domain protein 3 functions mechanically by binding to methylated DNA which is an essential step in regulating gene expression. It has a molecular mass of approximately 32 kDa. MBD3 is ubiquitously expressed in various tissues demonstrating its role in fundamental cellular processes. The protein does not function alone and often interacts with other proteins as part of gene repression mechanisms.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe activity of MBD3 impacts chromatin remodeling and gene expression regulation. MBD3 operates as a core component of the nucleosome remodeling deacetylase (NuRD) complex a multi-protein assembly important for transcriptional repression and chromatin structure modification. Its interaction within this complex helps modulate gene expression by altering the accessibility of chromatin and recruiting other modifiers for further regulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nMBD3 plays a significant role in key signaling and epigenetic pathways. It engages in pathways related to chromatin modification and transcription regulation. Its role in the NuRD complex underlines its participation in transcriptional regulation pathways impacting proteins such as HDAC1 and GATA2 which contribute to modifying histone acetylation and influencing gene transcription processes.\u003cbr\u003e\nChanges in MBD3 expression or function have links to cancer and neurodevelopmental disorders. MBD3 dysregulation associates with tumorigenesis due to its role in maintaining proper gene silencing and expression. Additionally impairment in MBD3 function can relate to intellectual disabilities and developmental delays potentially due to its interaction with epigenetic regulators like MeCP2.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851620962473,"sku":"ab240144","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab240144","provider":"Iright","version":"1.0","type":"link"}