{"product_id":"abcam-ab240191","title":"Abcam, ab240191, Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal ASH2L antibody. Carrier free. Suitable for IHC-P, WB, ICC\/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 2 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR13107(B),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:ICC\/IF, WB, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab240191 is the carrier-free version of\u003cbr\u003e\nab176334\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nASH2L also known as ASH2 is a protein with a mass of approximately 75 kDa. It functions as part of the trithorax group (TrxG) complex specifically the COMPASS-like complex where it plays an important role in histone methylation. This methylation occurs at histone H3 on lysine 4 (H3K4) an important modification for transcriptional activation. ASH2L expresses in various tissues with significant levels in the nucleus due to its involvement in chromatin modification.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nASH2L participates in the regulation of gene expression. It acts as a core subunit of the SET\/MLL family of H3K4 methyltransferases which form part of larger COMPASS-like complexes. These complexes modulate chromatin structure influencing transcriptional activation by methylating histone H3 at lysine 4. This modification is essential for the transition from transcriptional repression to activation during development and cell differentiation. ASH2L's role in these processes highlights its importance in maintaining proper gene expression patterns.\u003cbr\u003e\nPathways\u003cbr\u003e\nASH2L participates in the Wnt signaling pathway and influences the cell cycle. In the Wnt pathway ASH2L interacts with proteins such as β-catenin to modulate the transcription of target genes. It also affects the methylation status of promoters in various signaling pathways contributing to the regulation of gene expression involved in cell proliferation and differentiation. Additionally ASH2L is functionally connected to other proteins like MLL1 which shares involvement in chromatin remodeling and is important for epigenetic regulation.\u003cbr\u003e\nASH2L association with cancer and neurodevelopmental disorders is significant. Changes in ASH2L expression or function can lead to dysregulation of gene expression promoting oncogenesis or developmental abnormalities. In some cancers ASH2L's interaction with MLL can result in altered histone marks affecting tumor suppressor or oncogene expression. Besides cancer neurodevelopmental disorders like intellectual disability may have links to ASH2L due to its role in regulating genes involved in brain development. Understanding ASH2L's interactions and dysfunctions in these contexts provides insight into potential therapeutic approaches.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851618078889,"sku":"ab240191","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab240191","provider":"Iright","version":"1.0","type":"link"}