{"product_id":"abcam-ab240237","title":"Abcam, ab240237, Anti-PFKM + PFKL antibody [EPR11904] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal PFKM antibody. Carrier free. Suitable for ICC\/IF, Flow Cyt (Intra), WB and reacts with Human, Rat, Mouse, Recombinant full length protein - Human samples. Cited in 2 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR11904,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab240237 is the carrier-free version of\u003cbr\u003e\nab181064\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPFKM and PFKL are key isoforms of phosphofructokinase an enzyme that catalyzes the phosphorylation of fructose-6-phosphate to fructose-16-bisphosphate in glycolysis. PFKM also known as phosphofructokinase muscle type has a mass of approximately 85 kDa while PFKL or phosphofructokinase liver type is about 83 kDa. These isoforms express mainly in muscle and liver tissues respectively where they play a significant role in energy metabolism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPFKM and PFKL are important components of the glycolysis metabolic pathway. These enzymes form a multi-subunit complex called phosphofructokinase that regulates the rate-limiting step of glycolysis ensuring efficient energy production. This complex adapts to changes in cellular energy demands and environmental conditions therefore controlling glucose breakdown.\u003cbr\u003e\nPathways\u003cbr\u003e\nPFKM and PFKL are integral to glycolysis and gluconeogenesis processes essential for energy production and glucose homeostasis. PFKM closely interacts with hexokinase and pyruvate kinase in the glycolytic pathway ensuring continuation of the energy yield process. PFKL participates similarly with both aiding in maintaining the proper functioning of cellular metabolic activities.\u003cbr\u003e\nAbnormalities in PFKM and PFKL activity associate with glycogen storage disease type VII (Tarui's disease) and non-insulin-dependent diabetes mellitus. PFKM mutations lead to impaired glycolysis in muscle cells causing exercise intolerance and muscle cramps while PFKL variants can alter glucose metabolism contributing to diabetes development. These proteins' roles in diseases provide insight into potential therapeutic targets for metabolic disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844218179753,"sku":"ab240237","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab240237","provider":"Iright","version":"1.0","type":"link"}