{"product_id":"abcam-ab240968","title":"Abcam, ab240968, Anti-Protamine 2 antibody [EPR15738] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Protamine 2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR15738,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab240968 is the carrier-free version of\u003cbr\u003e\nab190791\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nProtamine 2 also known as PRM2 or protamine protein is a nuclear protein with a molecular mass of approximately 14 kDa. It is predominantly expressed in the testes specifically during the spermatogenesis process. Protamine 2 replaces histones in haploid phases of spermatids to ensure the proper condensation of sperm chromatin. Its structure comprises arginine-rich sequences enabling the tight packaging of DNA which is important for mature sperm functionality.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nProtamine 2 plays an essential role in sperm chromatin structure and stability interacting with other nucleoproteins to form a protamine-histone-DNA complex. This interaction is fundamental for the accurate packing of DNA within a sperm cell. Protamine 2 and its partners in the complex are critical for the fertility of males as they impact the genetic information's effective transmission to the next generation. The alteration of Protamine's balance affects the integrity of human sperm leading to reproductive challenges.\u003cbr\u003e\nPathways\u003cbr\u003e\nProtamine 2 integrates into the spermatogenesis pathway coordinating with other proteins like Protamine 1 and transition proteins. Together they conduct transitions from histone-based chromatin to protamine-based packaging. This process is essential within the broader context of the reproductive signaling pathway which includes a range of post-translational modifications central to sperm development and maturation.\u003cbr\u003e\nDisruptions in Protamine 2 expression link to male infertility as imbalances in Protamine 1 and 2 ratios can result in defective sperm packaging. Evidence also associates Protamine 2 dysregulation with a higher risk of sperm DNA fragmentation which can contribute to disorders such as recurrent pregnancy loss. The protein's performance and interaction with other proteins like histones and nuclear transition proteins point to its importance in maintaining genomic integrity during the paternal genetic material transmission.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844265169065,"sku":"ab240968","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab240968","provider":"Iright","version":"1.0","type":"link"}