{"product_id":"abcam-ab241000","title":"Abcam, ab241000, Phenylalanine Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nPhenylalanine Assay Kit (Colorimetric) ab241000 provides a quick, simple and accurate method for quantifying Phenylalanine in biological samples. Readout on any colorimetric (450 nm) plate reader. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Lysate, Serum, Cell Lysate,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhenylalanine Assay Kit (Colorimetric) (ab241000) provides a quick, simple and accurate method for quantifying Phenylalanine in biological samples. In the assay, Phenylalanine is metabolized with the simultaneous formation of NADH which reacts with a probe to generate absorbance that can be followed colorimetrically at 450 nm. The assay is linear in the range from 0.2 to 10 nmol, allowing accurate determination of concentrations in the range of 1 - 50 μM Phenylalanine.\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K481 Phenylalanine Assay Kit (Colorimetric). K481-100 is the same size as the 100 test size of ab241000.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPhenylalanine often abbreviated as Phe is an essential amino acid with a molecular mass of 165.19 g\/mol. It serves as a precursor for the biosynthesis of proteins and other compounds. Phenylalanine is transported into cells by a specific membrane transporter and expresses mainly in the liver and brain tissues. In laboratory settings phenylalanine assays and kits are often used to measure its concentration in biological samples which can be colorimetrically or fluorescently assessed.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPhenylalanine aids in the formation of proteins by incorporating into the polypeptide chains during ribosomal translation. It is not part of a molecular complex but acts as a free amino acid in various cellular processes. Its conversion to tyrosine by the enzyme phenylalanine hydroxylase is an important step in its metabolism. This conversion is necessary because tyrosine is used in the production of neurotransmitters and hormones.\u003cbr\u003e\nPathways\u003cbr\u003e\nPhenylalanine plays an important role in the phenylalanine and tyrosine catabolic pathway. This pathway is critical for the metabolism of aromatic amino acids eventually leading to the synthesis of important compounds like dopamine and norepinephrine. The enzyme phenylalanine hydroxylase closely related to this pathway shows the connection between phenylalanine and subsequent tyrosine-derived neurotransmitters illustrating its importance in neurochemical regulation.\u003cbr\u003e\nPhenylalanine is strongly associated with the genetic disorder phenylketonuria (PKU) resulting from mutations in the phenylalanine hydroxylase gene. This deficiency leads to high levels of phenylalanine in the blood causing neurotoxicity and cognitive impairments if left untreated. Management of PKU involves a diet low in phenylalanine connecting it to medical nutrition strategies. Furthermore high phenylalanine levels can affect proteins involved in neurotransmitter production linking it to potential neurological complications when not properly regulated.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843554332841,"sku":"ab241000","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab241000","provider":"Iright","version":"1.0","type":"link"}