{"product_id":"abcam-ab241002","title":"Abcam, ab241002, Deubiquitinase Assay Kit","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nDeubiquitinase Assay Kit (ab241002) provides a straight-forward and general measure of deubiquitinase activity by utilizing a fluorescent deubiquitinase substrate. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Cell culture extracts, Tissue Extracts,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nDeubiquitinase Assay Kit (ab241002) provides a straight-forward and general measure of deubiquitinase activity by utilizing a fluorescent deubiquitinase substrate to detect activity as low as 0.25 μU with purified enzyme.\u003cbr\u003e\nFl-Substrate -------\u0026gt; Cleaved Substrate + Fluorescence\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K485 Deubiquitinase Activity Assay Kit (Fluorometric). K485-100 is the same size as the 100 test size of ab241002.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDe-ubiquitinating enzymes (DUBs) function mechanically by removing ubiquitin moieties from proteins reversing what ubiquitination does. This action regulates protein degradation localization and activity. DUBs include enzymes such as ubiquitin-specific proteases (USPs) and ubiquitin C-terminal hydrolases (UCHs). These enzymes are typically expressed in various tissues throughout the body aligning with their roles in multiple cellular processes. DUB molecular masses vary widely reflecting the diverse structures and functions within this enzyme family.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nDe-ubiquitination regulates protein stability and function impacting processes like DNA repair cell cycle and signal transduction. DUBs may operate as part of a larger complex interfacing with other protein groups to maintain cellular equilibrium. They handle ubiquitin chains after proteins have been tagged for degradation critical for maintaining cellular proteostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nDe-ubiquitinating enzymes integrate into systems that oversee protein quality control and signal transduction. Key pathways include the ubiquitin-proteasome system and the endoplasmic reticulum-associated degradation (ERAD) pathway. Related proteins within these pathways include E3 ligases such as Mdm2 and substrate proteins like p53 as de-ubiquitination dictates their fates and manipulation in cellular environments.\u003cbr\u003e\nDe-ubiquitinating enzymes have roles in cancer and neurodegenerative diseases. Alterations in DUB activities link to disorders such as Parkinson's disease and various cancers through their role in protein regulation and signal pathways. For instance DUBs influence the stability of proteins like p53 in cancer and alpha-synuclein in Parkinson's disease pointing to their importance in disease progression and therapeutic targeting.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843644084393,"sku":"ab241002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab241002","provider":"Iright","version":"1.0","type":"link"}