{"product_id":"abcam-ab241031","title":"Abcam, ab241031, Monoamine Oxidase (MAO) Assay Kit","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nMonoamine Oxidase (MAO) Assay Kit (ab241031) is a sensitive assay to detect total monoamine oxidase activity as well as MAO-A and MAO-B isoenzyme activities separately in the presence of Clorgyline and Selegiline - specific inhibitors for MAO-A and MAOB, respectively. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells, Plasma, Tissue Homogenate, Serum\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMonoamine Oxidase (MAO) Assay Kit (ab241031) is a sensitive assay to quantify total monoamine oxidase activity. The kit can also be used to measure MAO-A and MAO-B isoenzyme activities separately by using the specific inhibitors for MAO-A (Clorgyline) and MAOB (Selegiline) that are included in the kit.\u003cbr\u003e\nThe assay is based on the fluorometric detection of H\u003cbr\u003e\n, one of the by-products generated during the oxidative deamination of the MAO substrate (Tyramine). The assay can detect as little as 5 μU of MAO enzymatic activity.\u003cbr\u003e\nRelated MAO assay kits\u003cbr\u003e\nUse this kit Monoamine Oxidase (MAO) Assay Kit ab241031 to measure total MAO, MAO-A and MAO-B activity in biological samples.\u003cbr\u003e\nUse Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric)\u003cbr\u003e\nab284510\u003cbr\u003e\n, and Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric)\u003cbr\u003e\nab284511\u003cbr\u003e\nto assay MAO inhibitors with purified MAO A and MAO B enzymes\u003cbr\u003e\nOther notes\u003cbr\u003e\nThis product was previously called K795 Biovision Monoamine Oxidase Activity (Total MAO\/MAO-A\/MAO-B) Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMonoamine oxidase (MAO) also known as MAO-A (with a mass of approximately 59 kDa) and MAO-B is a mitochondrial enzyme that catalyzes the oxidative deamination of monoamines. It primarily exists in the outer mitochondrial membrane and plays a significant role in the metabolism of neurotransmitters such as serotonin dopamine and norepinephrine. MAO enzymes are majorly found in the brain liver and gastrointestinal tract and their activity regulates neurotransmitter levels that influence mood and behavior.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMAO activity impacts the regulation of neurotransmitter signaling in the nervous system. MAO does not function as a part of a large complex; it exists as a dimeric protein yet independently performs critical enzymatic tasks. Its primary function is to prevent the accumulation of excessive monoamines which could lead to neurotoxicity and affect cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nMAO plays an important role in the catecholamine and serotonin signaling pathways. In particular it functions in degrading serotonin and catecholamines affecting neurotransmission balance. Through these pathways MAO interacts with other critical proteins such as catechol-O-methyltransferase (COMT) and dopamine beta-hydroxylase which also participate in the modulation of neurotransmitter activity.\u003cbr\u003e\nMAO influences several conditions including depression and Parkinson's disease. Alterations in MAO activity can affect levels of serotonin and other neurotransmitters linked to these disorders. Additionally in the context of neurological diseases changes in MAO function can interact with proteins like alpha-synuclein potentially contributing to neurodegenerative processes associated with Parkinson's disease.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843640414377,"sku":"ab241031","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab241031","provider":"Iright","version":"1.0","type":"link"}