{"product_id":"abcam-ab243268","title":"Abcam, ab243268, Recombinant human Ihh protein (Active)","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRecombinant human Ihh protein (Active) is a Human Fragment protein, in the 29 to 202 aa range, expressed in Escherichia coli, with \u0026gt;96%, \u0026lt; 1 EU\/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;96% SDS-PAGE,\u003cbr\u003e\nEndotoxin level:\u0026lt; 1 EU\/µg,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:HPLC, SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1\/2(CCL-226) cells is 3.0-10 μg\/ml.,\u003cbr\u003e\nAccession:Q14623,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Reconstitute in water or 0.1% BSA aqueous buffer,\u003cbr\u003e\nStorage buffer:Constituents: Phosphate Buffer, 0.87% Sodium chloride\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Indian hedgehog (Ihh) protein known by its gene symbol IHH plays key roles in cell signaling. Ihh with an approximate mass of 45 kDa belongs to the Hedgehog family of signaling molecules. It is expressed in various tissues including cartilage bone and parts of the gastrointestinal tract where it regulates growth and patterning. Ihh acts by binding to its receptor Patched triggering downstream signaling that influences cell fate decisions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nIhh is significant in regulating the differentiation and proliferation of chondrocytes in the growth plate of long bones. It acts as part of a complex regulatory network coordinating with Parathyroid hormone-related protein (PTHrP) to maintain proper development of endochondral bones. Ihh also influences epithelial and mesenchymal interactions during embryo development evidencing its important role in developmental biology.\u003cbr\u003e\nPathways\u003cbr\u003e\nIhh is a critical component of the Hedgehog signaling pathway a conserved pathway involved in embryonic development and adult tissue homeostasis. This pathway also includes proteins like Sonic hedgehog (Shh) and Desert hedgehog (Dhh) as well as the receptor Smoothened which mediates the signaling cascade. Ihh signaling impacts pathways such as the Wnt pathway influencing the proliferation and differentiation of cells thereby highlighting its integration into broader cellular signaling networks.\u003cbr\u003e\nIhh misregulation can lead to skeletal disorders like brachydactyly type A1 characterized by shortened fingers and toes. Furthermore abnormal Ihh signaling can contribute to certain cancers such as chondrosarcoma where overactive Hedgehog signaling promotes tumorigenesis. The relationship between Ihh and other Hedgehog proteins including Shh is key in understanding these pathologies as they share pathways that are often disrupted in disease scenarios.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843920973993,"sku":"ab243268","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab243268","provider":"Iright","version":"1.0","type":"link"}