{"product_id":"abcam-ab243298","title":"Abcam, ab243298, Tissue Clearing Kit - hydrophobic","description":"\u003cp\u003eSize: 15mL \/ 30mL\u003cbr\u003e\nTissue Clearing Kit ab243298 enables the easy set-up of rapid tissue clearing for 3D tissue imaging using simple protocols and standard laboratory equipment.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nTissue Clearing Kit - hydrophobic ab243298 enables the easy set-up of rapid tissue clearing for 3D tissue imaging using simple protocols and standard laboratory equipment.\u003cbr\u003e\nThe kit enables you to set up tissue clearing easily and clear tissue quickly. The protocols are simple and use standard laboratory equipment, and can be used with immunostaining, fluorescent proteins and chemical dyes. Clearing is reversible so that you can treat your sample for conventional H\u0026amp;E or IHC staining after 3D imaging.\u003cbr\u003e\nTo help you get started with tissue clearing, we have tissue clearing validated antibodies. Validated antibodies are for key markers, such as NeuN, GFAP, Iba1, etc, and are tested for tissue clearing with 1 mm mouse brain sections, and also for 3D cell culture clearing with neuronal spheroids and hepatic spheroids (depending on the marker).\u003cbr\u003e\nIf you want to set up tissue clearing on a sample type that we haven’t tested or to use your own antibodies, please consult the protocol book for this kit.\u003cbr\u003e\nTo learn more about tissue clearing, and tissue clearing methods (including for 3D cell cultures), please review our guide to tissue clearing.\u003cbr\u003e\nTissue clearing reagents and companion products are also available as individual products.\u003cbr\u003e\nThese tissue clearing reagents were developed with Visikol Inc, who developed the Visikol® HISTO™ tissue clearing technology which is the basis of our reagents.\u003cbr\u003e\nSimplified tissue clearing and immunostaining workflow:\u003cbr\u003e\n- fix tissue section in your preferred fixative\u003cbr\u003e\n- dehydrate with increasing % methanol series\u003cbr\u003e\n- incubate with DMSO\/methanol\u003cbr\u003e\n- rehydrate with decreasing % methanol series\u003cbr\u003e\n- incubate with Penetration Buffer\u003cbr\u003e\n- block with Blocking Buffer\u003cbr\u003e\n- stain with primary antibody, wash, stain with secondary antibody\u003cbr\u003e\n- dehydrate with increasing % methanol series\u003cbr\u003e\n- incubate with Tissue Clearing Reagent 1\u003cbr\u003e\n- incubate with Tissue Clearing Reagent 2\u003cbr\u003e\n- image tissue section\u003cbr\u003e\nThe full process, excluding fixation time and imaging, can be completed in ~ 3 days.\u003cbr\u003e\nThe workflow shown is for immuno-staining a 1 mm brain section, see kit protocol book for guidance for fluorescent proteins, labeling with chemical dyes, and other sample types \/ thicknesses.\u003cbr\u003e\nSuitable sample types\u003cbr\u003e\nThe tissue clearing reagents can be used with mammalian tissues of thicknesses ranging from conventional thin tissue sections up to whole organs.\u003cbr\u003e\nWhen starting to use tissue clearing, we recommend using sections of no more than 1 mm thick; at this thickness, protocols are shorter, there are less requirements on the equipment used for imaging, and the volume of data generated is less, so analysis is easier.\u003cbr\u003e\nWe recommend consulting the kit protocol book before collecting tissue samples.\u003cbr\u003e\nIf you are preparing 1 or 2 mm mouse brain sections, we recommend our Brain Slicers.\u003cbr\u003e\nImaging\u003cbr\u003e\nPlease consult the section on Imaging in the kit protocol book, before purchasing the kit. Confocal microscopes and light sheet microscopes are often suitable for 3D imaging of cleared tissues.\u003cbr\u003e\nIf you are using an air gap objective, you should also purchase a Silicon Imaging Chamber.\u003cbr\u003e\nIf you are using an immersion objective, you may require a double chambered cuvette.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847596855465,"sku":"ab243298","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab243298","provider":"Iright","version":"1.0","type":"link"}