{"product_id":"abcam-ab245100","title":"Abcam, ab245100, Anti-HE4 antibody [EPR16658-65] - BSA and Azide free (Detector)","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal HE4 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR16658-65,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab245100 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human HE4. The recommended pair for sandwich ELISA is:\u003cbr\u003e\nCapture:\u003cbr\u003e\nab244827\u003cbr\u003e\n, Human HE4 Capture Antibody (unconjugated)\u003cbr\u003e\nDetector: ab245100, Human HE4 Detector Antibody (unconjugated)\u003cbr\u003e\nThe reference range value is 23.4 - 2500 pg\/ml.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHuman Epididymis Protein 4 (HE4) also known as WAP four-disulfide core domain protein 2 (WFDC2) is a small glycoprotein of approximately 13 kDa. HE4 is most highly expressed in the epithelial cells of reproductive tissues like the epididymis but also in tissues such as the respiratory and urogenital tracts. HE4 is secreted into the extracellular space where it functions and is found in normal range in different body fluids. This secreted protein has gained attention due to its use in various diagnostic tests such as the HE4 test and HE4 assay utilizing HE4 antibodies.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHE4 participates in innate immune response and epithelial cell protection. HE4 protein plays a role in the modulation of antiprotease activity as it is involved in inhibiting serine proteases. It is not part of a larger protein complex but interacts with multiple protease signaling pathways. HE4 is often studied for its role in modulating protein activity at epithelial barriers contributing to tissue homeostasis and protective mechanisms against infections.\u003cbr\u003e\nPathways\u003cbr\u003e\nHE4 interacts within signaling cascades such as the innate immune pathways and extracellular matrix organization. It links to proteins within these pathways like serine proteases and other WFDC family proteins. HE4 influences protease activity regulation an essential component of tissue remodeling and inflammatory response impacting overall pathway dynamics.\u003cbr\u003e\nHE4 has strong associations with ovarian cancer and endometrial cancer. Its overexpression in these malignancies makes it a valuable biomarker for disease diagnosis and prognosis. HE4 interacts with cancer-related proteins such as CA125 enhancing its utility in combinatory diagnostic approaches. The HE4 test and HE4 ELISA kit price underline its significance in clinical settings for monitoring these cancers where timely detection influences treatment outcomes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851582197929,"sku":"ab245100","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab245100","provider":"Iright","version":"1.0","type":"link"}