{"product_id":"abcam-ab249798","title":"Abcam, ab249798, Anti-ATP4A antibody [EPR12251] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal ATP4A antibody. Carrier free. Suitable for WB and reacts with Human, Rat samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR12251,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Rat, Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab249798 is the carrier-free version of\u003cbr\u003e\nab174293\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nMouse: We have preliminary internal testing data to indicate this antibody may not react with this species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nATP4A also known as the gastric H+\/K+ ATPase alpha subunit is an important enzyme in the gastric lining. It is a P-type ATPase with a molecular mass of approximately 100 kDa. ATP4A primarily expresses in the parietal cells of the stomach where it contributes to the secretion of hydrochloric acid. This alpha subunit forms a catalytic core that cooperates with the beta subunit to actively transport ions across the stomach cell membrane.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nATP4A facilitates the acidification process necessary for digestion. It operates as part of the gastric proton pump complex. This complex exchanges K+ ions from the gastric lumen with H+ ions from within the parietal cell cytoplasm driving the generation of stomach acid. The enzyme's action is central to the maintenance of the acidic pH in the stomach which is important for proper protein digestion and absorption of various nutrients.\u003cbr\u003e\nPathways\u003cbr\u003e\nATP4A plays a major role in the gastric acid secretion pathway. This pathway includes interactions with the carbonic anhydrase enzyme which provides the necessary bicarbonate ions to initiate the acidification process. ATP4A also forms interactions with the Na+\/K+ ATPase as a part of ion balance and energy supply ensuring that the ionic gradients are appropriately established for gastric functions.\u003cbr\u003e\nATP4A's function is directly related to conditions like peptic ulcer disease and gastric cancer. Hyperactivity of ATP4A can lead to excessive acid secretion contributing to peptic ulcers. Additionally its chronic overexpression may have links to gastric cancer through persistent mucosal damage. Research also explores its association with other proteins like the epidermal growth factor receptor (EGFR) which can influence gastric tissue proliferation and healing processes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46852551868585,"sku":"ab249798","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab249798","provider":"Iright","version":"1.0","type":"link"}