{"product_id":"abcam-ab250017","title":"Abcam, ab250017, Anti-YAF2 antibody [EPR11020] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal YAF2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR11020,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody is not suitable to detect YAF2 in cell lines.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250017 is the carrier-free version of\u003cbr\u003e\nab177945\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nYAF2 also known as YY1AP1 is a protein that functions mechanically by interacting with other proteins to influence gene transcription. It has a molecular mass of approximately 19 kDa. YAF2 is expressed in a wide range of tissues; notable expression can be found in the nucleus where it can modulate the function of other nuclear factors. It associates particularly with regions of chromatin and is involved in the regulation of transcriptional activity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nYAF2 plays a role in regulating cell proliferation and apoptosis. It is part of the polycomb group complex which modifies chromatin structure and directly impacts gene expressivity. YAF2 interfaces with proteins like YY1 functioning as a transcription cofactor. It modulates gene expression by either repressing or enhancing transcription depending on the context and specific gene targets being acted upon.\u003cbr\u003e\nPathways\u003cbr\u003e\nThis protein is involved in the Wnt signaling pathway and the cell cycle regulation pathway. In the Wnt signaling pathway YAF2 influences the transcriptional regulation of target genes by impacting cellular growth and differentiation processes. It is also related to proteins within these pathways such as β-catenin in the Wnt pathway where it can potentially modulate downstream effects. Through regulating the cell cycle YAF2 impacts the transition between different cell cycle stages interfacing with proteins like cyclins that control progression through the cell cycle.\u003cbr\u003e\nAlterations in YAF2 activity relate to cancer and developmental disorders. Aberrant expression or mutations in YAF2 have been linked to oncogenic processes particularly in the context of its interactions with YY1 which is a known factor involved in various cancers. Additionally disruptions in its role within the polycomb group complex may contribute to developmental impairments affecting how gene silencing is orchestrated during embryogenesis. Further investigations into YAF2 and its interactions could provide insights into therapeutic targets for these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851926818985,"sku":"ab250017","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab250017","provider":"Iright","version":"1.0","type":"link"}