{"product_id":"abcam-ab250179","title":"Abcam, ab250179, Anti-Tripeptidyl peptidase II\/TPPII antibody [EPR13202] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Tripeptidyl peptidase II\/TPPII antibody. Carrier free. Suitable for IHC-P, ICC\/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR13202,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), IHC-P, WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250179 is the carrier-free version of\u003cbr\u003e\nab180177\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTripeptidyl peptidase II (TPPII) also known as TPP2 is a large peptidase complex that mechanistically degrades oligopeptides into tripeptides. It consists of a massive molecular complex with a molecular mass of approximately 5-6 MDa. TPPII is ubiquitously expressed in various tissues with high expression levels in the liver muscle and kidney. This complex is cytosolic and possesses unique endopeptidase and exopeptidase activities which makes it distinct in both structure and function from the proteasome.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTPPII is an enzyme that plays a vital role in the regulation of intracellular protein turnover and antigen processing. It contributes to protein degradation especially when proteasome activities are inhibited or overwhelmed. TPPII operates in a complex and involves the dynamic assembly of its subunits to form a functional peptidase complex. Through its action TPPII maintains protein homeostasis and assists in generating peptides presented by MHC class I molecules important for immune surveillance.\u003cbr\u003e\nPathways\u003cbr\u003e\nTPPII functions within the ubiquitin-proteasome system and the antigen presentation pathway. It acts as a backup to the proteasome ensuring continued protein degradation. TPPII interacts with proteins such as ubiquitin and E3 ligases which tag proteins for degradation. During antigen processing TPPII complements proteasome activity by processing longer peptides into shorter ones suitable for MHC class I presentation thereby interfacing with molecules like TAP and the proteasome subunits.\u003cbr\u003e\nDysregulation of TPPII is associated with conditions such as cancer and neurodegenerative diseases. The upregulation of TPPII has been observed in several tumors implicating its role in promoting cell survival and proliferation. In cancer TPPII may interact with proteins such as p53 affecting apoptosis and cell cycle regulation. In neurodegenerative diseases altered TPPII function might influence protein aggregation and degradation pathways where it might relate with proteins like tau and amyloid-beta contributing to disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851777134761,"sku":"ab250179","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab250179","provider":"Iright","version":"1.0","type":"link"}