{"product_id":"abcam-ab250319","title":"Abcam, ab250319, Anti-Seladin 1 antibody [EPR10743] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Seladin 1 antibody. Carrier free. Suitable for WB and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR10743,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250319 is the carrier-free version of\u003cbr\u003e\nab181062\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSeladin 1 also known as DHCR24 (3β-Hydroxysteroid-Δ24 reductase) is an enzyme with a molecular mass of approximately 54 kDa. It plays a role in cholesterol biosynthesis by catalyzing the reduction of a double bond at the C-24 position in sterol precursors. Seladin 1 is expressed in various tissues with high expression in the brain liver and adrenal gland. The enzyme contributes to the maintenance of cellular cholesterol homeostasis and provides neuroprotective functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSeladin 1 plays an important role in cellular processes particularly in response to oxidative stress. The protein is not part of a larger complex but functions independently to reduce highly reactive oxygen species levels in cells. It helps in maintaining cellular integrity and contributes to the survival of neuronal cells under stress conditions. Additionally it provides resistance to oxidative stress-induced apoptosis by modulating intracellular antioxidative defense mechanisms.\u003cbr\u003e\nPathways\u003cbr\u003e\nSeladin 1 plays an integral role in the cholesterol biosynthesis pathway and is closely associated with the mevalonate pathway. Its activity directly influences the availability of cholesterol and other sterols impacting membrane fluidity and cellular functions dependent on sterol concentrations. The enzyme interacts with related proteins such as HMG-CoA reductase an important regulatory enzyme within the cholesterol synthesis pathway. These interactions ensure that cellular cholesterol levels are well-regulated to meet physiological demands.\u003cbr\u003e\nSeladin 1 has been implicated in Alzheimer's disease and certain cancers. In Alzheimer's disease a reduction in Seladin 1 expression correlates with increased susceptibility to amyloid-beta toxicity while its overexpression shows a protective effect. This relationship ties Seladin 1 to amyloid precursor protein processing. In some cancers altered Seladin 1 expression affects tumor growth and progression where it may interact with proteins involved in cell survival pathways. This dual role in both neurodegenerative and cancerous conditions highlights the importance of Seladin 1 in human health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851261202601,"sku":"ab250319","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab250319","provider":"Iright","version":"1.0","type":"link"}