{"product_id":"abcam-ab250674","title":"Abcam, ab250674, Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal CDK1 phospho T14 antibody. Carrier free. Suitable for IP, Dot, WB, IHC-P and reacts with Human, Mouse, Rat, Synthetic peptide samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR17499,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:IP, IHC-P, WB, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250674 is the carrier-free version of\u003cbr\u003e\nab183550\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCdk1 and Cdk2 also known as cyclin-dependent kinase 1 and cyclin-dependent kinase 2 are essential enzymes in the regulation of the cell cycle. These kinases play important roles in controlling the cell's transition through different stages of the cell cycle. Cdk1 has a molecular mass of about 34 kDa while Cdk2 weighs approximately 33 kDa. Both are expressed in various tissues with high expression levels found in proliferating cells. These kinases require binding to specific cyclins to activate their kinase activities which then phosphorylate target substrates involved in cell cycle progression.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCdks are pivotal in driving cell cycle phases collaborating with cyclins to regulate checkpoints at G1\/S and G2\/M transitions. Cdk1 primarily associates with cyclin B to facilitate the entry into mitosis whereas Cdk2 partners with cyclin E and cyclin A to advance the G1\/S transition. Their activity is indispensable for proper cell cycle regulation and any misregulation can lead to uncontrolled cell proliferation. Importantly their involvement in complex formation ensures orderly progression and coordination across cell cycle stages.\u003cbr\u003e\nPathways\u003cbr\u003e\nCdk1 and Cdk2 integrate into the cell cycle regulation pathways and the DNA damage response pathways. In the cell cycle pathway Cdks are required for the orderly progression and checkpoints that ensure proper mitotic entry and DNA synthesis. Similarly in response to DNA damage these kinases work closely with proteins like p21 which can inhibit Cdk activity to halt cell cycle progression and facilitate DNA repair. By coordinating with related proteins like cyclins and p21 Cdk1 and Cdk2 help maintain genomic integrity through effective cell cycle control.\u003cbr\u003e\nCdk1 and Cdk2 have significant implications in cancer particularly as their overactivity often leads to tumorigenesis. Aberrations in Cdk-cyclin activity disrupt normal cell cycle control contributing to unchecked cell division seen in cancers. Moreover Cdk2 has connections to neurodegenerative diseases where its deregulation may contribute to neuronal cell death. These kinases relate to proteins such as p53 in cancer pathways and have become targets for pharmacological inhibition to restore controlled cell growth in cancer therapy.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46856181055657,"sku":"ab250674","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab250674","provider":"Iright","version":"1.0","type":"link"}