{"product_id":"abcam-ab250871","title":"Abcam, ab250871, Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal RYBP antibody. Carrier free. Suitable for IP, ChIP, WB, IHC-P, ICC\/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR13059(2),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), ChIP, IHC-P, IP, ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab250871 is the carrier-free version of\u003cbr\u003e\nab185971\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRYBP also known as Ring1 and YY1 Binding Protein has a critical mechanical role in gene regulation. It has a molecular weight of approximately 28 kDa. It is a part of the Polycomb group (PcG) proteins which take part in chromatin remodeling to regulate gene expression. RYBP is found in the nucleus of cells and is known to bind DNA directly. Its expression occurs widely across various tissues indicating its broad involvement in cellular functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein actively participates in maintaining transcriptional repression through its involvement in the Polycomb Repressive Complex 1 (PRC1). PRC1 includes other key proteins such as Ring1A\/B and CBX proteins which work together to modulate chromatin structure. RYBP contributes to the ubiquitination of histone H2A a process essential for maintaining gene silencing. This activity is important during developmental stages influencing cell fate decisions and lineage commitment.\u003cbr\u003e\nPathways\u003cbr\u003e\nRYBP plays an essential role in the regulation of the Wnt signaling pathway and the apoptosis pathway. Within the Wnt pathway RYBP acts in part by interacting with proteins such as β-catenin influencing cell proliferation and differentiation. In the apoptosis pathway it is involved through its interactions with E3 ubiquitin ligases potentially affecting cell death regulation. These pathways showcase how RYBP integrates into important cellular processes impacting cellular homeostasis.\u003cbr\u003e\nRYBP has connections to cancer and neurodevelopmental disorders. Its altered expression is linked to tumor progression where it interacts with other PcG proteins like EZH2 often showing overexpression in cancers. These interactions can lead to abnormal gene silencing contributing to oncogenesis. In neurodevelopmental contexts disruptions in RYBP's function are associated with changes in neural differentiation and might correlate with disorders like autism spectrum disorders highlighting its broader relevance beyond typical gene expression roles.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851632890025,"sku":"ab250871","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab250871","provider":"Iright","version":"1.0","type":"link"}