{"product_id":"abcam-ab251399","title":"Abcam, ab251399, Anti-MTHFR antibody [EPR19781] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal MTHFR antibody. Carrier free. Suitable for ICC\/IF, IP, WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR19781,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:IP, ICC\/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab251399 is the carrier-free version of\u003cbr\u003e\nab203786\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe 'MTHFR' protein also known as methylenetetrahydrofolate reductase plays an important role in folate metabolism by catalyzing the reduction of 510-methylenetetrahydrofolate to 5-methyltetrahydrofolate. This enzyme has a mass of approximately 70 kDa and is expressed in various tissues including liver kidney and brain. It exists primarily in the cytoplasm where it performs its enzymatic functions critical for cellular metabolism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMethylenetetrahydrofolate reductase is essential in the process of transferring one-carbon units especially in the synthesis of the amino acid methionine and the methylation of DNA. This enzyme does not form part of a larger protein complex but operates independently in the folate cycle. Its activity directly influences homocysteine levels within the cell with alterations in its function affecting many metabolic processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nMethylenetetrahydrofolate reductase is an important component of the folate and methionine cycles significant in the remethylation of homocysteine to methionine. These pathways intersect with transmethylation and nucleotide synthesis essential for cell functions and replication. It interacts with proteins like methionine synthase and cystathionine beta-synthase which further demonstrate the enzyme's importance in maintaining cellular homeostasis.\u003cbr\u003e\nMethylenetetrahydrofolate reductase deficiency or mutations often lead to hyperhomocysteinemia and have been associated with cardiovascular diseases and neurological disorders. Mutations in this enzyme can alter its efficiency leading to the accumulation of homocysteine. This has connections with proteins such as methionine synthase where impaired functioning due to MTHFR mutation can exacerbate the disruption of homocysteine metabolism contributing to disease states.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851641835689,"sku":"ab251399","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab251399","provider":"Iright","version":"1.0","type":"link"}