{"product_id":"abcam-ab251553","title":"Abcam, ab251553, Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Anterior Gradient 2 antibody. Carrier free. Suitable for WB, ICC\/IF, Flow Cyt (Intra) and reacts with Mouse, Recombinant fragment - Human, Human, Rat samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR20164-222,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:ICC\/IF, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab251553 is the carrier-free version of\u003cbr\u003e\nab216071\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAGR2 (anterior gradient protein 2) and AGR3 (anterior gradient protein 3) are small proteins with roles in cellular homeostasis. AGR2 has a mass of approximately 20 kDa while AGR3 is around 18 kDa. Both proteins localize in the endoplasmic reticulum (ER) and are expressed notably in epithelial tissues like the gastrointestinal tract and respiratory epithelium. AGR2 also known as PDIA17 functions as a protein disulfide isomerase which assists in protein folding. AGR3 shares structural similarities with AGR2 suggesting overlapping functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAGR2 and AGR3 influence processes such as cell growth and repair. They are necessary for maintaining the proper folding of secreted and membrane proteins. AGR2 interacts with mucin proteins and this complex formation is important for mucin production which supports protective mucus barriers. AGR3's specific biological partners are less well-defined but it complements AGR2's functions in similar tissue contexts suggesting it might share similar interactions.\u003cbr\u003e\nPathways\u003cbr\u003e\nAGR2 and AGR3 engage in pathways that link ER function to broader cellular activities. One important pathway involves the unfolded protein response (UPR) where AGR2 helps mitigate ER stress by ensuring proper protein assembly and folding. Additionally AGR2 participates in the ERK signaling pathway impacting cell proliferation and differentiation. Proteins such as ATF6 and GRP78 interact with AGR2 in these pathways indicating its role in stress response and growth signaling networks.\u003cbr\u003e\nAGR2 and AGR3 gain attention in relation to cancer and cystic fibrosis. In cancer especially breast and prostate AGR2 overexpression correlates with tumor progression and metastasis. It interacts with proteins like MUC1 and HER2 facilitating oncogenic pathways. AGR3 while less studied shows similar trends suggesting potential involvement. In cystic fibrosis AGR2 associates with compromised mucin production and secretion causing exacerbated lung conditions. CXCL8 a mucin-related protein is seen alongside AGR2 activity in these inflammatory scenarios.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851572400297,"sku":"ab251553","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab251553","provider":"Iright","version":"1.0","type":"link"}